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Literature summary for 3.4.21.68 extracted from

  • Nabiabad, H.S.; Piri, K.; Amini, M.
    Expression of active chimeric-tissue plasminogen activator in tobacco hairy roots, identification of a DNA aptamer and purification by aptamer functionalized-MWCNTs chromatography (2018), Protein Expr. Purif., 152, 137-145 .
    View publication on PubMed
Search for more literature for 3.4.21.68

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of active chimeric mutant of tissue plasminogen activator in Nicotiana tabacum hairy roots Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the activity of tPA is increased by splicing the active site of dodder-cuscutain gene to human tPA. The chimeric cDNA of tPA is constructed through splicing by overlap extension PCR (SOEing-PCR) method and transferred to the hairy roots of tobacco using different strains of Agrobacterium rhizogenes. Agrobacterium rhizogenes strain ATCC 15834 is the most favorable strain for the induction of hairy roots in tobacco, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Chi-tPA 1 specific aptamers are designed using SELEX method, possesses high affinity for the chimeric mutant enzyme Homo sapiens
Chi-tPA 2 specific aptamers are designed using SELEX method, possesses good affinity for the chimeric mutant enzyme Homo sapiens
Chi-tPA 3 specific aptamers are designed using SELEX method, possesses good affinity for the chimeric mutant enzyme Homo sapiens
Chi-tPA 4 specific aptamers are designed using SELEX method, possesses good affinity for the chimeric mutant enzyme Homo sapiens
Chi-tPA 5 specific aptamers are designed using SELEX method, possesses good affinity for the chimeric mutant enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00750
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant chimeric tissue plasminogen activator mutant from Nicotiana tabacum hairy roots by lysine affinity chromatography. In addition, specific aptamers are designed using SELEX method. Multi wall carbon nanotubes are functionalized with selected aptamers, Chi-tPA1-5, packed in a column, and used for purification. The selected aptamer Chi-tPA1, having KD values of 0.320 nM and IC50 of 28.9 nM, possesses good affinity to tPA, and the chimeric mutant tPA is properly purified by aptamer-chromatography. Development of aptamer-based affinity purification method, overview Homo sapiens

Subunits

Subunits Comment Organism
? x * 62000, recombinant chimeric mutant enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
Tissue-type plasminogen activator
-
 Homo sapiens
tPA
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.5 assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000223
-
 pH 7.4-8.5, 37°C, recombinant chimeric enzyme Homo sapiens Chi-tPA 2
0.0000289
-
 pH 7.4-8.5, 37°C, recombinant chimeric enzyme Homo sapiens Chi-tPA 1
0.000197
-
 pH 7.4-8.5, 37°C, recombinant chimeric enzyme Homo sapiens Chi-tPA 3
0.0002
-
 pH 7.4-8.5, 37°C, recombinant chimeric enzyme Homo sapiens Chi-tPA 4
0.00021
-
 pH 7.4-8.5, 37°C, recombinant chimeric enzyme Homo sapiens Chi-tPA 5

General Information

General Information Comment Organism
physiological function tissue-type plasminogen activator (tPA) is a serine protease that plays a crucial role in the fibrinolytic system Homo sapiens