Literature summary for 3.4.21.68 extracted from

Long, X.; Gou, Y.; Luo, M.; Zhang, S.; Zhang, H.; Bai, L.; Wu, S.; He, Q.; Chen, K.; Huang, A.; Zhou, J.; Wang, D.
Soluble expression, purification, and characterization of active recombinant human tissue plasminogen activator by auto-induction in E. coli (2015), BMC Biotechnol., 15, 13 .

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Application

Application	Comment	Organism
medicine	tissue plasminogen activator converts plasminogen into plasmin and is used clinically to treat thrombosis	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
gene PLAT, functional expression of His-tagged soluble full-length tPA, that contains multiple disulfide bonds and the RGDS, as the integrin binding motif, fused in C-terminus, on an industrial scale using autoinduction in Escherichia coli strains BL21 (DE3), Rosetta, and Origami 2, method optimization and evaluation, overview. Strain Origami 2 can increase disulfide bond formation in cytoplasmic tPA and produce purified soluble recombinant protein	Homo sapiens

Cloned (Comment)

Organism

gene PLAT, functional expression of His-tagged soluble full-length tPA, that contains multiple disulfide bonds and the RGDS, as the integrin binding motif, fused in C-terminus, on an industrial scale using autoinduction in Escherichia coli strains BL21 (DE3), Rosetta, and Origami 2, method optimization and evaluation, overview. Strain Origami 2 can increase disulfide bond formation in cytoplasmic tPA and produce purified soluble recombinant protein

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	the RGDS motif is fused to the enzyme's C-terminus. RGDS, as the binding motif of integrin, improves the specific affinity of the recombinant tPA to substrate. The similar diameter of the halo patterns of His-tag-PSP-tPA-RGDS and tPA-RGDS suggests that they exhibit similar amidolytic activities in vitro	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60800	-	recombinant soluble detagged enzyme, gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the enzyme converts plasminogen into plasmin	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00750	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged soluble full-length enzyme tPA from Escherichia coli by nickel affinity chromatography, the His-tag is cleaved off by PreScission Protease (PSP), leaving only two additional residues (Gly and Pro) at the N-terminus, followed by gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme converts plasminogen into plasmin	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 65000, recombinant soluble detagged enzyme, SDS-PAGE, 1 * 63800, about, sequence calculation	Homo sapiens
More	the recombinant protease exists mostly as a monomer under native conditions rather than in an intermolecular disulfide-bonded oligomeric form	Homo sapiens

Synonyms

Synonyms	Comment	Organism
PLAT	-	Homo sapiens
Tissue plasminogen activator	-	Homo sapiens
tPA	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	human tissue plasminogen activator belongs to the serine protease family	Homo sapiens