Literature summary for 3.4.21.68 extracted from

Rahimpour, A.; Vaziri, B.; Moazzami, R.; Nematollahi, L.; Barkhordari, F.; Kokabee, L.; Adeli, A.; Mahboudi, F.
Engineering the cellular protein secretory pathway for enhancement of recombinant tissue plasminogen activator expression in Chinese hamster ovary cells: effects of CERT and XBP1s genes (2013), J. Microbiol. Biotechnol., 23, 1116-1122.

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Application

Application	Comment	Organism
medicine	recombinant human t-PA is extensively used as a therapeutic agent for the treatment of thrombotic diseases owing to its greater safety and efficiency compared with other plasminogen activators like urokinase and streptokinase. CERTS132A-based secretion engineering can be an effective strategy for enhancing recombinant enzyme production in CHO cells	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant overexpression of the enzyme in CHO ATCC CRL-9606 cells and secretion to the cell culture medium, co-expression with CERT S132A, a mutant form of CERT that is resistant to phosphorylation, and XBP1s, method optimization and evaluation, overview. Overexpression of CERT S132A increases the specific productivity of t-PA-producing CHO cells up to 35%, while the heterologous expression of XBP1s does not affect the t-PA expression rate	Homo sapiens

Cloned (Comment)

Organism

recombinant overexpression of the enzyme in CHO ATCC CRL-9606 cells and secretion to the cell culture medium, co-expression with CERT S132A, a mutant form of CERT that is resistant to phosphorylation, and XBP1s, method optimization and evaluation, overview. Overexpression of CERT S132A increases the specific productivity of t-PA-producing CHO cells up to 35%, while the heterologous expression of XBP1s does not affect the t-PA expression rate

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68000	-	x * 68000, recombinant enzyme, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
plasminogen + H2O	Homo sapiens	activation	plasmin + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00750	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
plasminogen + H2O	activation	Homo sapiens	plasmin + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 68000, recombinant enzyme, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
t-PA	-	Homo sapiens
Tissue plasminogen activator	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	the human tissue plasminogen activator is a serine protease known as the key component of the fibrinolytic system. t-PA converts the proenzyme plasminogen to plasmin, which in turn mediates the degradation of fibrin	Homo sapiens