Literature summary for 3.4.21.68 extracted from

Luo, X.G.; Tian, W.J.; Ni, M.; Jing, X.L.; Lv, L.H.; Wang, N.; Jiang, Y.; Zhang, T.C.
Soluble expression of active recombinant human tissue plasminogen activator derivative (K2S) in Escherichia coli (2011), Pharm. Biol., 49, 653-657.

Search for more literature for 3.4.21.68

Application

Application	Comment	Organism
medicine	the kringle 2 plus serine protease domains, K2S, of human tissue plasminogen activator is an efficacious thrombolytic drug, which is used to treat heart attacks and strokes by breaking up the fibrin clots that cause them	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
high-level expression of the kringle 2 plus serine protease domains, K2S, of human tissue plasminogen activator in Escherichia coli strain BL21 as active fusion protein, in which the His-tagged K2S domains are fused to the disulfide isomerase DsbC from Escherichia coli, the recombinant fusion protein is soluble. The active K2S domains are liberated by cleavage through factor Xa. Subcloning in Escherichia coli strain DH5alpha	Homo sapiens

Cloned (Comment)

Organism

high-level expression of the kringle 2 plus serine protease domains, K2S, of human tissue plasminogen activator in Escherichia coli strain BL21 as active fusion protein, in which the His-tagged K2S domains are fused to the disulfide isomerase DsbC from Escherichia coli, the recombinant fusion protein is soluble. The active K2S domains are liberated by cleavage through factor Xa. Subcloning in Escherichia coli strain DH5alpha

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
plasminogen activator inhibitor-1	astrocyte-derived	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged kringle 2 plus serine protease domains, K2S, of human tissue plasminogen activator from Escherichia coli by nickel affinity chromatography after cleavage with factor Xa	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	assay on fibrinolytic activity	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	the enzyme contains 9 disulfide bridges required for proper folding	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Tissue plasminogen activator	-	Homo sapiens
tPA	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
additional information	production of active K2S in Escherichia coli without the requirements of in vitro refolding process, method optimization, overview	Homo sapiens