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Literature summary for 3.4.21.68 extracted from

  • Zhang, H.; Bi, F.; Xiao, C.; Liu, J.; Wang, Z.; Liu, J.N.; Zhang, J.
    The Kringle-2 domain of tissue plasminogen activator significantly reduces mortality and brain infarction in middle cerebral artery occlusion rats (2010), Int. J. Mol. Med., 26, 225-230.
    View publication on PubMed
Search for more literature for 3.4.21.68

Protein Variants

Protein Variants Comment Organism
additional information reteplase is a bacterially expressed TPA deletion mutant comprising residues 176-527 of wild-type TPA. Plasminogen is activated to plasmin by reteplase, and then reteplase is completely cleaved by plasmin into a two-chain form, which is composed of Kringle-2 and P domain linked by a disulfide bond Rattus norvegicus
S478A non-proteolytic TPA full-length mutant Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
plasminogen + H2O Rattus norvegicus
-
 plasmin + ?
-
 ?
plasminogen + H2O Rattus norvegicus Sprague-Dawley
-
 plasmin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus Sprague-Dawley
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O endogenous TPA activities in the ipsilateral ischemic hemisphere are measured using casein-zymography Rattus norvegicus ?
-
 ?
casein + H2O endogenous TPA activities in the ipsilateral ischemic hemisphere are measured using casein-zymography Rattus norvegicus Sprague-Dawley ?
-
 ?
plasminogen + H2O
-
 Rattus norvegicus plasmin + ?
-
 ?
plasminogen + H2O
-
 Rattus norvegicus Sprague-Dawley plasmin + ?
-
 ?

Synonyms

Synonyms Comment Organism
Alteplase
-
 Rattus norvegicus
More alteplase is the full-length recombinant human TPA, while reteplase (K2P) is the domain deletion mutant comprising only the Kringle-2 domain and protease domain of TPA Rattus norvegicus
Tissue plasminogen activator
-
 Rattus norvegicus
tPA
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Rattus norvegicus

General Information

General Information Comment Organism
additional information alteplase is the full-length recombinant human TPA, while reteplase, K2P, is the domain deletion mutant comprising only the Kringle-2 domain and protease domain of TPA. Reteplase shows a better protective effect than alteplase, suggesting that F, P, or K1 domain in TPA diminishes the brain-protective effect Rattus norvegicus
physiological function the Kringle-2 domain, residues 176-262 of TPA, is the essential structure required for the brain-protective activity of TPA, employing the rat middle cerebral artery occlusion, MCAO, model. The Kringle-2 domain of tissue plasminogen activator significantly reduces mortality and brain infarction in middle cerebral artery occlusion rats. Tissue plasminogen activator shows brainprotective activity within the first 15 min after cerebral ischemia in rats Rattus norvegicus