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Literature summary for 3.4.21.68 extracted from
- Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells (2009), Blood, 113, 470-478.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| plasminogen activator inhibitor-1 | dose-dependently facilitates the dissociation of membrane-retained tPA and increases the amounts of tPA-plasminogen activator inhibitor-1 high-molecular-weight complexes in the medium | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subcloned into NheI/BamHI sites in GFP3.1 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S478A | efficacy of expression of the mutant and wild-type is similar, but the mutant does not appear in the culture medium after 24 h either complexed with plasminogen activator inhibitor-1 or in the free form. Intracellular distribution is indistinguishable to the wild-type, but it is present at higher concentrations than the wild-type near the plasma membrane | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | exocytosed tPA-GFP is retained at the membrane surface without retrieval for more than 5 min in a vascular endothelial cell-specific manner. FnEK1K2 domains are responsible for binding of tPA-GFP to the membrane surface | Homo sapiens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 147000 | - |
by Western blot analysis or by anti-GFP antibody, tPA-GFP-plasminogen activator inhibitor-1 complex | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| EAhy 926 cell | vascular endothelial cell line producing GFP-tagged tPA | Homo sapiens | - |
| granule cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glu-plasminogen + H2O | - |
Homo sapiens | plasmin + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tissue plasminogen activator | - |
Homo sapiens |
| tPA | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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