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Literature summary for 3.4.21.67 extracted from

  • Goldberg, A.L.; Swamy, K.H.S.; Chung, C.H.; Larimore, F.S.
    Proteases in Escherichia coli (1981), Methods Enzymol., 80, 680-702.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
DFP
-
Escherichia coli
additional information not: metal chelating agents; sulfhydryl inhibitors; tosyllysine chloromethyl ketone Escherichia coli
Pentanamide
-
Escherichia coli
PMSF
-
Escherichia coli
tosylphenylalanine chloromethyl ketone
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Escherichia coli 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
2 * 70000, SDS-PAGE Escherichia coli
140000
-
E. coli, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Methyl-alpha-casein + H2O
-
Escherichia coli Hydrolyzed methyl-alpha-casein
-
?
Methylhemoglobin + H2O
-
Escherichia coli Hydrolyzed methylhemoglobin
-
?

Subunits

Subunits Comment Organism
dimer 2 * 70000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Escherichia coli