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Literature summary for 3.4.21.66 extracted from
- Production and purification of novel thermostable alkaline protease from Anoxybacillus sp. KP1 (2015), Cell. Mol. Biol. (Noisy-le-grand), 61, 113-120 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Anoxybacillus sp. KP1 |  |
| Cu2+ | - |
Anoxybacillus sp. KP1 | |
| Triton X-100 | - |
Anoxybacillus sp. KP1 | |
| Tween 80 | - |
Anoxybacillus sp. KP1 | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Anoxybacillus sp. KP1 | |
| H2O2 | - |
Anoxybacillus sp. KP1 | |
| Hg2+ | - |
Anoxybacillus sp. KP1 | |
| additional information | the presence of detergents, the protease is quite stable and residual activity lays between 73% and 82% | Anoxybacillus sp. KP1 | |
| PMSF | complete inhibition | Anoxybacillus sp. KP1 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Anoxybacillus sp. KP1 | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anoxybacillus sp. KP1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme from strain KP1 by ammonium sulfate fractionation and gel filtration | Anoxybacillus sp. KP1 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | KP1 is a thermopholic and aerobic strain. Among the carbon and nitrogen sources used, the optimum protease production is obtained with soluble starch, maltose, urea and casamino acid | Anoxybacillus sp. KP1 | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 106000, SDS-PAGE | Anoxybacillus sp. KP1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alkaline protease | - |
Anoxybacillus sp. KP1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Anoxybacillus sp. KP1 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | purified native enzyme, stable at | Anoxybacillus sp. KP1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Anoxybacillus sp. KP1 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme is a serine alkaline protease | Anoxybacillus sp. KP1 |
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Release 2023.1 (January 2023)
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