Cloned (Comment) | Organism |
---|---|
recombinant expression in Lactococcus lactis strain P170 from vector pAMJ2008 and in strain MG1363, secretion of stable thermitase in an auto-induced fermentation setup at 30°C. The enzyme accumulates in the culture supernatant during batch fermentations and is easily activated at 50°C or by prolonged dialysis | Laceyella sacchari |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Laceyella sacchari |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
8500 | - |
recombinant enzyme, gel filtration, thermitase interactions with the column matrix can delay elution of the protein and lead to underestimation of the molecular size | Laceyella sacchari |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Laceyella sacchari | - |
- |
- |
Laceyella sacchari DSM 43353 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from culture supernatant by dialysis and hydrophobic interaction chromatography | Laceyella sacchari |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1483 | - |
purified recombinant enzyme, pH 8.0, 60°C | Laceyella sacchari |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Laceyella sacchari | ? | - |
? | |
azocasein + H2O | - |
Laceyella sacchari DSM 43353 | ? | - |
? | |
additional information | a thermostable endo-protease with the ability to convert various food relevant substrates into low-molecular weight peptides, endo-catalytic activity | Laceyella sacchari | ? | - |
? | |
additional information | a thermostable endo-protease with the ability to convert various food relevant substrates into low-molecular weight peptides, endo-catalytic activity | Laceyella sacchari DSM 43353 | ? | - |
? | |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Laceyella sacchari | N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Laceyella sacchari DSM 43353 | N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Laceyella sacchari |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | activity range, recombinant enzyme | Laceyella sacchari |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme, stable up to | Laceyella sacchari |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Laceyella sacchari |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | activity range, recombinant enzyme | Laceyella sacchari |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | purified recombinant enzyme, 50°C, 5 h, over 60% activity | Laceyella sacchari |