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Literature summary for 3.4.21.66 extracted from

  • Hanphakphoom, S.; Maneewong, N.; Sukkhum, S.; Tokuyama, S.; Kitpreechavanich, V.
    Characterization of poly(L-lactide)-degrading enzyme produced by thermophilic filamentous bacteria Laceyella sacchari LP175 (2014), J. Gen. Appl. Microbiol., 60, 13-22.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic tree Laceyella sacchari

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline at 5 mM Laceyella sacchari
2-mercaptoethanol slight inhibition Laceyella sacchari
Ca2+ 18% inhibition at 1 mM, 85% inhibition at 4 mM Laceyella sacchari
CTAB
-
Laceyella sacchari
Cu2+ 8% inhibition at 1 mM, 99% inhibition at 4 mM Laceyella sacchari
DTT slight inhibition at 1-10 mM Laceyella sacchari
EDTA strong inhibition at 1 mM Laceyella sacchari
EGTA strong inhibition at 1 mM Laceyella sacchari
Fe2+ 54% inhibition at 1 mM, 80% inhibition at 4 mM Laceyella sacchari
K+ 36% inhibition at 1 mM, 53% inhibition at 4 mM Laceyella sacchari
Mg2+ 54% inhibition at 1 mM, 89% inhibition at 4 mM Laceyella sacchari
additional information inhibition by metal ions is blocked by EDTA Laceyella sacchari
Na+ 35% inhibition at 1 mM, 54% inhibition at 4 mM Laceyella sacchari
Plysurf A210G strong inhibition Laceyella sacchari
PMSF strong inhibition at 1 mM Laceyella sacchari
SDS strong inhibition Laceyella sacchari
Triton X-100
-
Laceyella sacchari
Tween 20 strong inhibition Laceyella sacchari
Tween 80 strong inhibition Laceyella sacchari
Urea slight inhibition Laceyella sacchari
Zn2+ 65% inhibition at 1 mM, 81% inhibition at 4 mM Laceyella sacchari

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Laceyella sacchari
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates to 119% at 1 mM Laceyella sacchari

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration Laceyella sacchari

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Laceyella sacchari the enzyme degrades poly(L-lactide) and is a serine protease ?
-
?
additional information Laceyella sacchari LP175 the enzyme degrades poly(L-lactide) and is a serine protease ?
-
?

Organism

Organism UniProt Comment Textmining
Laceyella sacchari
-
isolated from forest soil in Thailand
-
Laceyella sacchari LP175
-
isolated from forest soil in Thailand
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 15.3fold to homogeneity from culture supernatant by dialysis, cation exchange chromatography, and again dialysis Laceyella sacchari

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
262
-
purified native enzyme, substrate gelatin, pH 9.0, 60°C Laceyella sacchari
328
-
purified native enzyme, substrate N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide, pH 9.0, 60°C Laceyella sacchari
340
-
purified native enzyme, substrate casein, pH 9.0, 60°C Laceyella sacchari

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O strong activity Laceyella sacchari ?
-
?
casein + H2O strong activity Laceyella sacchari LP175 ?
-
?
Gelatin + H2O strong activity Laceyella sacchari ?
-
?
Gelatin + H2O strong activity Laceyella sacchari LP175 ?
-
?
additional information substrate specificity, overview Laceyella sacchari ?
-
?
additional information the enzyme degrades poly(L-lactide) and is a serine protease Laceyella sacchari ?
-
?
additional information substrate specificity, overview Laceyella sacchari LP175 ?
-
?
additional information the enzyme degrades poly(L-lactide) and is a serine protease Laceyella sacchari LP175 ?
-
?
N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O weak activity Laceyella sacchari ?
-
?
N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O weak activity Laceyella sacchari LP175 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Laceyella sacchari

Synonyms

Synonyms Comment Organism
PLLA-degrading enzyme
-
Laceyella sacchari
thermostable serine protease
-
Laceyella sacchari

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Laceyella sacchari

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified native enzyme, stable up to Laceyella sacchari

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Laceyella sacchari

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5 10.5 purified native enzyme, stable at Laceyella sacchari