Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree | Laceyella sacchari |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | at 5 mM | Laceyella sacchari | |
2-mercaptoethanol | slight inhibition | Laceyella sacchari | |
Ca2+ | 18% inhibition at 1 mM, 85% inhibition at 4 mM | Laceyella sacchari | |
CTAB | - |
Laceyella sacchari | |
Cu2+ | 8% inhibition at 1 mM, 99% inhibition at 4 mM | Laceyella sacchari | |
DTT | slight inhibition at 1-10 mM | Laceyella sacchari | |
EDTA | strong inhibition at 1 mM | Laceyella sacchari | |
EGTA | strong inhibition at 1 mM | Laceyella sacchari | |
Fe2+ | 54% inhibition at 1 mM, 80% inhibition at 4 mM | Laceyella sacchari | |
K+ | 36% inhibition at 1 mM, 53% inhibition at 4 mM | Laceyella sacchari | |
Mg2+ | 54% inhibition at 1 mM, 89% inhibition at 4 mM | Laceyella sacchari | |
additional information | inhibition by metal ions is blocked by EDTA | Laceyella sacchari | |
Na+ | 35% inhibition at 1 mM, 54% inhibition at 4 mM | Laceyella sacchari | |
Plysurf A210G | strong inhibition | Laceyella sacchari | |
PMSF | strong inhibition at 1 mM | Laceyella sacchari | |
SDS | strong inhibition | Laceyella sacchari | |
Triton X-100 | - |
Laceyella sacchari | |
Tween 20 | strong inhibition | Laceyella sacchari | |
Tween 80 | strong inhibition | Laceyella sacchari | |
Urea | slight inhibition | Laceyella sacchari | |
Zn2+ | 65% inhibition at 1 mM, 81% inhibition at 4 mM | Laceyella sacchari |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Laceyella sacchari | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates to 119% at 1 mM | Laceyella sacchari |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Laceyella sacchari |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Laceyella sacchari | the enzyme degrades poly(L-lactide) and is a serine protease | ? | - |
? | |
additional information | Laceyella sacchari LP175 | the enzyme degrades poly(L-lactide) and is a serine protease | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Laceyella sacchari | - |
isolated from forest soil in Thailand | - |
Laceyella sacchari LP175 | - |
isolated from forest soil in Thailand | - |
Purification (Comment) | Organism |
---|---|
native enzyme 15.3fold to homogeneity from culture supernatant by dialysis, cation exchange chromatography, and again dialysis | Laceyella sacchari |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
262 | - |
purified native enzyme, substrate gelatin, pH 9.0, 60°C | Laceyella sacchari |
328 | - |
purified native enzyme, substrate N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide, pH 9.0, 60°C | Laceyella sacchari |
340 | - |
purified native enzyme, substrate casein, pH 9.0, 60°C | Laceyella sacchari |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | strong activity | Laceyella sacchari | ? | - |
? | |
casein + H2O | strong activity | Laceyella sacchari LP175 | ? | - |
? | |
Gelatin + H2O | strong activity | Laceyella sacchari | ? | - |
? | |
Gelatin + H2O | strong activity | Laceyella sacchari LP175 | ? | - |
? | |
additional information | substrate specificity, overview | Laceyella sacchari | ? | - |
? | |
additional information | the enzyme degrades poly(L-lactide) and is a serine protease | Laceyella sacchari | ? | - |
? | |
additional information | substrate specificity, overview | Laceyella sacchari LP175 | ? | - |
? | |
additional information | the enzyme degrades poly(L-lactide) and is a serine protease | Laceyella sacchari LP175 | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O | weak activity | Laceyella sacchari | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O | weak activity | Laceyella sacchari LP175 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Laceyella sacchari |
Synonyms | Comment | Organism |
---|---|---|
PLLA-degrading enzyme | - |
Laceyella sacchari |
thermostable serine protease | - |
Laceyella sacchari |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Laceyella sacchari |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified native enzyme, stable up to | Laceyella sacchari |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Laceyella sacchari |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10.5 | purified native enzyme, stable at | Laceyella sacchari |