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Literature summary for 3.4.21.63 extracted from

  • Yilmaz, B.; Baltaci, M.O.; Sisecioglu, M.; Adiguzel, A.
    Thermotolerant alkaline protease enzyme from Bacillus licheniformis A10 purification, characterization, effects of surfactants and organic solvents (2016), J. Enzyme Inhib. Med. Chem., 31, 1241-1247 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 94% activation at 1 mM, 70% at 5 mM Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
acetone loss of 64-74% activity at 15-50% v/v after 24 h, loss of 27-70% activity after 1 h Bacillus licheniformis
Ag+ about 19% inhibition at 1-10 mM Bacillus licheniformis
butanol loss of 40-68% activity at 15-50% v/v after 24 h, loss of 40-47% activity after 1 h Bacillus licheniformis
chloroform loss of 45-88.5% activity at 15-50% v/v after 24 h, loss of 31-21% activity after 1 h Bacillus licheniformis
Co2+ 8% inhibition at 10 mM Bacillus licheniformis
DMSO loss of 41-53% activity at 15-50% v/v after 24 h, loss of 21-48% activity after 1 h Bacillus licheniformis
DTNB 52-57% inhibition at 1-5 mM Bacillus licheniformis
EDTA complete inhibition at 5 mM, 50% at 1 mM Bacillus licheniformis
ethanol loss of 55-78% activity at 15-50% v/v after 24 h, loss of 8-55% activity after 1 h Bacillus licheniformis
Fe2+ 45% inhibition at 10 mM, 33.5% at 5 mM Bacillus licheniformis
formaldehyde loss of 27-13% activity at 15-50% v/v after 24 h, loss of 46-47% activity after 1 h Bacillus licheniformis
H2O2 73-74% inhibition at 1-5 mM Bacillus licheniformis
Isopropanol loss of 39-45% activity at 15-50% v/v after 24 h, loss of about 50% activity after 1 h Bacillus licheniformis
methanol loss of 75-100% activity at 15-50% v/v after 24 h, loss of 44-93% activity after 1 h Bacillus licheniformis
Ni2+ 32% inhibition at 10 mM, 28% at 5 mM Bacillus licheniformis
PMSF complete inhibition at 5 mM, 97% at 1 mM Bacillus licheniformis
SDS complete inhibition at 5 mM, 96% at 1 mM Bacillus licheniformis
Triton X-100 45% inhibition at 5 mM, 39% at 1 mM Bacillus licheniformis
Tween 20 66% inhibition at 5 mM, 39% at 1 mM Bacillus licheniformis
Tween 80 72% inhibition at 1-5 mM Bacillus licheniformis
Urea 71% inhibition at 1 mM, 95.5% at 8 mM Bacillus licheniformis
Zn2+ 40% inhibition at 10 mM, 38% at 1-5 mM Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km and Vmax values of the protease enzyme purified from strain A10 are 0.033 mg/ml and 8.17 mmol/ml/min, respectively, with casein as substrate Bacillus licheniformis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus licheniformis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates 15% at 10 mM Bacillus licheniformis
Mg2+ activates 5% at 10 mM Bacillus licheniformis
Mn2+ activates 14.5% at 10 mM Bacillus licheniformis
additional information no effect on activity by Ca2+ Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
originally isolated from Erzurum Ilica hot springs
-
Bacillus licheniformis A10
-
originally isolated from Erzurum Ilica hot springs
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 1.38fold from strain A10 by ammonium sulfate fractionation, dialysis, and anion exchange chromatography Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O 97% activity compared to casein Bacillus licheniformis ?
-
?
azocasein + H2O 97% activity compared to casein Bacillus licheniformis A10 ?
-
?
Bovine serum albumin + H2O 18% activity compared to casein Bacillus licheniformis ?
-
?
Bovine serum albumin + H2O 18% activity compared to casein Bacillus licheniformis A10 ?
-
?
casein + H2O the enzyme has a high affinity for the casein, best substrate Bacillus licheniformis ?
-
?
casein + H2O the enzyme has a high affinity for the casein, best substrate Bacillus licheniformis A10 ?
-
?
Gelatin + H2O 28% activity compared to casein Bacillus licheniformis ?
-
?
Gelatin + H2O 28% activity compared to casein Bacillus licheniformis A10 ?
-
?
Hemoglobin + H2O 60% activity compared to casein Bacillus licheniformis ?
-
?
Hemoglobin + H2O 60% activity compared to casein Bacillus licheniformis A10 ?
-
?

Subunits

Subunits Comment Organism
? x * 40550, extracellular enzyme, SDS-PAGE Bacillus licheniformis

Synonyms

Synonyms Comment Organism
Alkaline protease
-
Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 over 70% of maximal activity within this range, profile overview Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 80 purified native extracellular enzyme, over 50% activity remaining after 15-30 min. After 60-min incubation, the enzyme preserves 70.74%, 54.22%, 94.82%, and 98.47% of its activity at 40°C, 50°C, 60°C, and 70°C, respectively Bacillus licheniformis
60 70 purified native extracellular enzyme, 1 h, 90% activity remaining Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.392
-
casein pH 8.0, 37°C Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
6 11 activity range, 70% of maximal activity at pH 6.0, 60% at pH 11.0, profile overview Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
purified native extracellular enzyme, 40% of activity remaining Bacillus licheniformis
7.5 10.5 purified native extracellular enzyme, over 60% activity remianing within this range Bacillus licheniformis
11
-
purified native extracellular enzyme, 30% of activity remaining Bacillus licheniformis

General Information

General Information Comment Organism
additional information the enzyme is a serine protease Bacillus licheniformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.392
-
casein pH 8.0, 37°C Bacillus licheniformis