Application | Comment | Organism |
---|---|---|
food industry | the enzyme is efficient in producing antihypertensive peptide IPP from beta-casein and a potential debittering agent. The high degree of hydrolysis of the enzyme to soybean protein (8.8%) and peanut protein (11.1%) compared to papain and alcalase makes it a good candidate in the processing of oil industry byproducts | Aspergillus oryzae |
Cloned (Comment) | Organism |
---|---|
recombinant expression of a truncated neutral protease I in Pichia pastoris with a high enzyme yield | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Aspergillus oryzae | |
EDTA | - |
Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the enzyme has a zinc-binding motif and is a gluzincin | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows a high degree of hydrolysis with soybean protein (8.8%) and peanut protein (11.1%) compared to papain and alcalase | Aspergillus oryzae | ? | - |
? | |
Oxidized insulin B-chain + H2O | eight cleavage sites of the enzyme in oxidized insulin B-chain are determined by mass spectrometry, and five of them have high hydrophobic amino acid affinity | Aspergillus oryzae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutral protease I | - |
Aspergillus oryzae |
NpI | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant truncated neutral protease I, 120 min, over 90% activity remaining | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | purified recombinant truncated neutral protease I, stable at | Aspergillus oryzae |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the gluzincin family | Aspergillus oryzae |