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Literature summary for 3.4.21.63 extracted from

  • Behnsen, J.; Lessing, F.; Schindler, S.; Wartenberg, D.; Jacobsen, I.D.; Thoen, M.; Zipfel, P.F.; Brakhage, A.A.
    Secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins C3, C4, and C5 (2010), Infect. Immun., 78, 3585-3594.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.63

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain BL21(DE3) Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
additional information an alp1 deletion strain, DELTAalp1, is generated from strain DELTAakuBKU80, and the culture supernatant possesses minimal complement-degrading activity. Moreover, protein extract derived from an Escherichia coli strain overproducing Alp1 cleaves C3b, C4b, and C5 Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted enzyme, large amounts in the culture supernatant Aspergillus fumigatus
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus fumigatus secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins in culture supernatant C3, C4, C5, and C1q as well as immunoglobulin G. C4 is completely degraded. Degradation of C5 and C1q is also observed but is less prominent ?
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 ?
additional information Aspergillus fumigatus DELTAakuBKU80 secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins in culture supernatant C3, C4, C5, and C1q as well as immunoglobulin G. C4 is completely degraded. Degradation of C5 and C1q is also observed but is less prominent ?
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 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
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Aspergillus fumigatus DELTAakuBKU80
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins in culture supernatant C3, C4, C5, and C1q as well as immunoglobulin G. C4 is completely degraded. Degradation of C5 and C1q is also observed but is less prominent Aspergillus fumigatus ?
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 ?
additional information secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins in culture supernatant C3, C4, C5, and C1q as well as immunoglobulin G. C4 is completely degraded. Degradation of C5 and C1q is also observed but is less prominent Aspergillus fumigatus DELTAakuBKU80 ?
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 ?

Synonyms

Synonyms Comment Organism
protease Alp1
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 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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 assay at Aspergillus fumigatus

General Information

General Information Comment Organism
physiological function secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins C3, C4, and C5. C3b is an important opsonin, but other complement proteins also have essential functions in the defense against microbes. C5b, a cleavage product of C5, initiates formation of the membrane attack complex at a later stage of complement activation, and C5a represents a potent anaphylatoxin. C1q initiates the classical pathway of complement activation, and C4 is part of the C3 convertase of the classical pathway. The enzyme is involved in the mechanism that contributes to evasion from the host complement attack, overview Aspergillus fumigatus