Cloned (Comment) | Organism |
---|---|
high-level expression of plasmids pPIC9K-n/Alp and pPIC9K-alpha/Alp in Pichia pastoris GS115 with native signal peptide or alpha-factor secretion signal peptide | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
benzamidine | slightly inhibits recombinant and native enzyme | Aspergillus oryzae | |
Cu2+ | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
EDTA | slightly inhibits recombinant and native enzyme | Aspergillus oryzae | |
Hg2+ | strong inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
Mn2+ | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
N-tosyl-L-lysine chloromethyl ketone | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
phenylmethylsulfonyl fluoride | strong inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
Zn2+ | inhibitor of recombinant and native enzyme | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates recombinant and native enzyme | Aspergillus oryzae | |
Mg2+ | stimulates recombinant and native enzyme | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, recombinant enzyme, SDS-PAGE | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | P12547 | strain Y2942 | - |
Aspergillus oryzae RIB 40 | P12547 | strain Y2942 | - |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate precipitation, ion exchange chromatography and gel filtration | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40.94 | - |
culture supernatant | Aspergillus oryzae |
92.73 | - |
purified recombinant enzyme | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Aspergillus oryzae | hydrolyzed casein | - |
? | |
casein + H2O | - |
Aspergillus oryzae RIB 40 | hydrolyzed casein | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, recombinant enzyme, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
Alkaline protease | - |
Aspergillus oryzae |
ALP | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant and native enzyme | Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | recombinant and native enzyme | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the native enzyme exhibits a better thermostability than the recombinant enzyme | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant and native enzyme | Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | recombinant and native enzyme | Aspergillus oryzae |