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Literature summary for 3.4.21.63 extracted from
- High-level expression, purification and characterization of recombinant Aspergillus oryzae alkaline protease in Pichia pastoris (2008), Protein Expr. Purif., 58, 301-308.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| high-level expression of plasmids pPIC9K-n/Alp and pPIC9K-alpha/Alp in Pichia pastoris GS115 with native signal peptide or alpha-factor secretion signal peptide | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | strong inhibitor of recombinant and native enzyme | Aspergillus oryzae |  |
| benzamidine | slightly inhibits recombinant and native enzyme | Aspergillus oryzae | |
| Cu2+ | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| EDTA | slightly inhibits recombinant and native enzyme | Aspergillus oryzae | |
| Hg2+ | strong inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| Mn2+ | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| N-tosyl-L-lysine chloromethyl ketone | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| phenylmethylsulfonyl fluoride | strong inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| Zn2+ | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates recombinant and native enzyme | Aspergillus oryzae | |
| Mg2+ | stimulates recombinant and native enzyme | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, recombinant enzyme, SDS-PAGE | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | P12547 | strain Y2942 | - |
| Aspergillus oryzae RIB 40 | P12547 | strain Y2942 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ammonium sulfate precipitation, ion exchange chromatography and gel filtration | Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40.94 | - |
culture supernatant | Aspergillus oryzae |
| 92.73 | - |
purified recombinant enzyme | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Aspergillus oryzae | hydrolyzed casein | - |
? | |
| casein + H2O | - |
Aspergillus oryzae RIB 40 | hydrolyzed casein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, recombinant enzyme, SDS-PAGE | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alkaline protease | - |
Aspergillus oryzae |
| ALP | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant and native enzyme | Aspergillus oryzae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | recombinant and native enzyme | Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the native enzyme exhibits a better thermostability than the recombinant enzyme | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
recombinant and native enzyme | Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | recombinant and native enzyme | Aspergillus oryzae |
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