Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.62 extracted from

  • Price, M.A.; Cruz, R.; Baxter, S.; Escalettes, F.; Rosser, S.J.
    CRISPR-Cas9 in situ engineering of subtilisin E in Bacillus subtilis (2019), PLoS ONE, 14, e0210121 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.62

Cloned(Commentary)

Cloned (Comment) Organism
gene aprE, genetic structure, recombinant expression of wild-type and mutant enzymes in Bacillus subtilis strain 168 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information CRISPR-Cas9 in situ engineering of subtilisin E in Bacillus subtilis by usage of a flexible, co-transformation approach where the single guide RNA is inserted in a plasmid for Cas9 co-expression, and the donor DNA is supplied as a linear PCR product observing an editing efficiency of 76%. The method allows multiple, rapid rounds of in situ editing of the subtilisin E gene to incorporate a salt bridge triad present in the Bacillus clausii thermotolerant homologue, M-protease. Method, overview. The enzyme mutant obtained shows increased thermotolerance and activity Bacillus subtilis
Q125/Q377E/Q381R site-directed mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme, but is 60% less active than the wild-type Bacillus subtilis
Q125R site-directed mutagenesis, the mutant does not show increased thermotolerance compared to the wild-type enzyme, it is 60% less active than the wild-type Bacillus subtilis
Q377E site-directed mutagenesis, the mutant shows increased thermotolerance and an increase in protease activity of 46.5% compared to the wild-type enzyme Bacillus subtilis
Q381R site-directed mutagenesis, the mutant shows increased thermotolerance and an increase in protease activity of 46.5% compared to the wild-type enzyme Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P04189
-
-
Bacillus subtilis 168 P04189
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Bacillus subtilis ?
-
 ?
casein + H2O
-
 Bacillus subtilis 168 ?
-
 ?

Synonyms

Synonyms Comment Organism
aprE
-
 Bacillus subtilis
Subtilisin E
-
 Bacillus subtilis

General Information

General Information Comment Organism
additional information the salt-bridge triad Gln125-Gln377-Gln381 in subtilisin E contributes to thermostability and activity. The Q125 residue is important for catalytic activity Bacillus subtilis