Literature summary for 3.4.21.62 extracted from

Falus, P.; Cerioli, L.; Bajnoczi, G.; Boros, Z.; Weiser, D.; Nagy, J.; Tessaro, D.; Servi, S.; Poppe, L.
A continuous-flow cascade reactor system for subtilisin A-catalyzed dynamic kinetic resolution of N-tert-butyloxycarbonylphenylalanine ethyl thioester with benzylamine (2016), Adv. Synth. Catal., 358, 1608-1617 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	the enzyme subtilisin A is immobilized by simple hydrophobic adsorption onto various surface-grafted macroporous silica gels resulting in easy-to-prepare and stable biocatalysts enabling the efficient kinetic resolution (KR) and dynamic kinetic resolution (DKR) of racemic N-tert-butyloxycarbonylphenylalanine ethyl thioester with benzylamine	Bacillus licheniformis

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Bacillus licheniformis	-

Storage Stability

Storage Stability	Organism
effect of one year storage on activity and selectivity of 23 different Alcalase preparations in the kinetic resolution of racemic N-tert-butyloxycarbonylphenylalanine ethyl thioester with benzylamine, overview	Bacillus licheniformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 rac-N-tert-butoxycarbonylphenylalanine ethyl thioester + 1-phenylmethanamine + tert-butanol	continuous-flow cascade reactor system for subtilisin A-catalyzed dynamic kinetic resolution (DKR). The continuous-mode DKR of the racemic thioester in a serial cascade system of six biocatalyst-filled columns at 50°C for KR and five grafted silica gel-filled columns at 150°C for racemization results in the formation of the (S)-benzylamide in 79% conversion, 8.17 g/l/h volumetric productivity and 98% enantiomeric excess. Method evaluation, overview	Bacillus licheniformis	N-benzyl-Nalpha-(tert-butoxycarbonyl)-L-phenylalaninamide + (R)-N-tert-butoxycarbonylphenylalanine ethyl thioester + ?	-	?

Synonyms

Synonyms	Comment	Organism
Alcalase	-	Bacillus licheniformis
subtilisin A	-	Bacillus licheniformis

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Release 2023.1 (January 2023)