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Literature summary for 3.4.21.62 extracted from

  • Martinez, D.E.; Borniego, M.L.; Battchikova, N.; Aro, E.M.; Tyystjaervi, E.; Guiamet, J.J.
    SASP, a senescence-associated subtilisin protease, is involved in reproductive development and determination of silique number in Arabidopsis (2015), J. Exp. Bot., 66, 161-174.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene At3g14067, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in an enzyme-knockout line sasp-1 or SALK_147962 and functional complementation Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q0WWH7 ecotype Col-0, gene At3g14067
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Posttranslational Modification

Posttranslational Modification Comment Organism
additional information occurrence of the two variant forms of AtSASP can be due to posttranslational modifications Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf mRNA levels and enzyme activity increase during leaf senescence in leaves senescing during both the vegetative or the reproductive phase of the plant life cycle, but this increase is more pronounced in reproductive plants Arabidopsis thaliana
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additional information the enzyme is expressed in all aboveground organs, but not in roots Arabidopsis thaliana
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Subunits

Subunits Comment Organism
? x * 59000-61000, zymogen enzyme form, DS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
SASP
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Arabidopsis thaliana
senescence-associated subtilisin protease
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Arabidopsis thaliana

General Information

General Information Comment Organism
evolution phylogenetic analysis, AtSASP and its putative orthologues clustering in one discrete group of subtilisin proteases in which no other Arabidospsis subtilisin protease is present. The enzyme function is at least partially conserved between Arabidopsis thaliana and Oryza sativa Arabidopsis thaliana
malfunction at maturity, enzyme knockout sasp-1 plants produce 25% more inflorescence branches and siliques, mostly due to an increased number of second and third order branches, than either the wild-type or the rescued lines Arabidopsis thaliana
additional information occurrence of the two variant forms of AtSASP can be due to posttranslational modifications Arabidopsis thaliana
physiological function the enzyme downregulates branching and silique production during monocarpic senescence Arabidopsis thaliana