Cloned (Comment) | Organism |
---|---|
gene At3g14067, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in an enzyme-knockout line sasp-1 or SALK_147962 and functional complementation | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q0WWH7 | ecotype Col-0, gene At3g14067 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | occurrence of the two variant forms of AtSASP can be due to posttranslational modifications | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | mRNA levels and enzyme activity increase during leaf senescence in leaves senescing during both the vegetative or the reproductive phase of the plant life cycle, but this increase is more pronounced in reproductive plants | Arabidopsis thaliana | - |
additional information | the enzyme is expressed in all aboveground organs, but not in roots | Arabidopsis thaliana | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000-61000, zymogen enzyme form, DS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
SASP | - |
Arabidopsis thaliana |
senescence-associated subtilisin protease | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis, AtSASP and its putative orthologues clustering in one discrete group of subtilisin proteases in which no other Arabidospsis subtilisin protease is present. The enzyme function is at least partially conserved between Arabidopsis thaliana and Oryza sativa | Arabidopsis thaliana |
malfunction | at maturity, enzyme knockout sasp-1 plants produce 25% more inflorescence branches and siliques, mostly due to an increased number of second and third order branches, than either the wild-type or the rescued lines | Arabidopsis thaliana |
additional information | occurrence of the two variant forms of AtSASP can be due to posttranslational modifications | Arabidopsis thaliana |
physiological function | the enzyme downregulates branching and silique production during monocarpic senescence | Arabidopsis thaliana |