Literature summary for 3.4.21.62 extracted from

Gallagher, T.; Ruan, B.; London, M.; Bryan, M.A.; Bryan, P.N.
Structure of a switchable subtilisin complexed with a substrate and with the activator azide (2009), Biochemistry, 48, 10389-10394.

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Cloned(Commentary)

Cloned (Comment)	Organism
subtilisin variants expressed in Escherichia coli	Bacillus amyloliquefaciens

Crystallization (Commentary)

Crystallization (Comment)	Organism
1.8 A resolution structure of an inactive form (by replacing the catalytic nucleophile Ser 221 with alanine) of the protease subtilisin S189, in complex with azide and with a prodomain substrate that spans the active site. The substrate is well ordered across the active site, and the azide anion is observed bound adjacent to Ala 32. Although S189, like wild-type subtilisin, has Ser as the catalytic nucleophile at residue 221, these crystal structures have Ala 221 to prevent cleavage of the substrate	Bacillus amyloliquefaciens

Crystallization (Comment)

Organism

1.8 A resolution structure of an inactive form (by replacing the catalytic nucleophile Ser 221 with alanine) of the protease subtilisin S189, in complex with azide and with a prodomain substrate that spans the active site. The substrate is well ordered across the active site, and the azide anion is observed bound adjacent to Ala 32. Although S189, like wild-type subtilisin, has Ser as the catalytic nucleophile at residue 221, these crystal structures have Ala 221 to prevent cleavage of the substrate

Bacillus amyloliquefaciens

Protein Variants

Protein Variants	Comment	Organism
A1C	does not change the catalytic properties of subtilisin S189 but allows the introduction of a fluorescent group at its N-terminus	Bacillus amyloliquefaciens
D32A	engineered variant of the protease subtilisin, denoted S189, mutation renders the enzyme's activity dependent on the presence of certain small anions such as fluoride or azide. Is activated more than 3000fold by azide	Bacillus amyloliquefaciens
S221A	inactive form of subtilisin S189	Bacillus amyloliquefaciens

Organism

Organism	UniProt	Comment	Textmining
Bacillus amyloliquefaciens	P00782	-	-

Purification (Commentary)

Purification (Comment)	Organism
by affinity purification, more than 95% pure	Bacillus amyloliquefaciens

Synonyms

Synonyms	Comment	Organism
subtilisin	-	Bacillus amyloliquefaciens