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Literature summary for 3.4.21.62 extracted from

  • Sung, J.H.; Ahn, S.J.; Kim, N.Y.; Jeong, S.K.; Kim, J.K.; Chung, J.K.; Lee, H.H.
    Purification, molecular cloning, and biochemical characterization of subtilisin JB1 from a newly isolated Bacillus subtilis JB1 (2010), Appl. Biochem. Biotechnol., 162, 900-911.
    View publication on PubMed
Search for more literature for 3.4.21.62

Application

Application Comment Organism
industry subtilisin JB1 may serve as a potential source material for use in industrial applications of proteolytic enzymes and microorganisms for fishery waste degradation and fish by-product processing Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
prosubtilisin JB1 cloned into the GST fusion protein expression vector pGEX-4T-1 and transformed into Escherichia coli DH5alphaMCR Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 40% Bacillus subtilis
antipain at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 75% Bacillus subtilis
Brij 35 at 37°C and pH of 7.5, 0.01% reduces prosubtilisin JB1 relative activity to 30% and 0.05% reduces prosubtilisin JB1 relative activity to 70% Bacillus subtilis
Ca2+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 57% and 5 mM reduces prosubtilisin JB1 relative activity to 45% Bacillus subtilis
chymostatin at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 95% Bacillus subtilis
Co2+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 14% and 5 mM reduces prosubtilisin JB1 relative activity to 24% Bacillus subtilis
Cu2+ at 37°C and pH of 7.5, with 1 mM results in an almost complete reduction of prosubtilisin JB1 activity, 5 mM reduces prosubtilisin JB1 relative activity to 19% Bacillus subtilis
E-64 at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 31% Bacillus subtilis
E-64c at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 25% Bacillus subtilis
EDTA at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 52% Bacillus subtilis
EGTA at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 34% Bacillus subtilis
Hg+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 33% and 5 mM reduces prosubtilisin JB1 relative activity to 22% Bacillus subtilis
K+ at 37°C and pH of 7.5, with 1 mM results in an almost complete reduction of prosubtilisin JB1 activity, 5 mM reduces prosubtilisin JB1 relative activity to 53% Bacillus subtilis
Mg2+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 79% and 5 mM reduces prosubtilisin JB1 relative activity to 48% Bacillus subtilis
additional information at 37°C and pH of 7.5, 0.1 mM leupeptin does not inhibit prosubtilisin JB1 Bacillus subtilis
N-ethylmaleimide at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 15% Bacillus subtilis
pepstatin A at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 48% Bacillus subtilis
phenylmethylsulfonyl fluoride at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 79% Bacillus subtilis
Sodium dodecyl sulfate at 37°C and pH of 7.5, 0.01% reduces prosubtilisin JB1 relative activity to 52% and 0.05% reduces prosubtilisin JB1 relative activity to 29% Bacillus subtilis
Triton X-100 at 37°C and pH of 7.5, 0.01% reduces prosubtilisin JB1 relative activity to 92% and 0.05% reduces prosubtilisin JB1 relative activity to 69% Bacillus subtilis
Tween 20 at 37°C and pH of 7.5, 0.01% reduces prosubtilisin JB1 relative activity to 53% and 0.05% reduces prosubtilisin JB1 relative activity to 70% Bacillus subtilis
Zn2+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 30%, with 5 mM results in an almost complete reduction of prosubtilisin JB1 activity Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 purified prosubtilisin JB1, Western blotting analysis Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis B6VFQ8 JB1
-
Bacillus subtilis JB1 B6VFQ8 JB1
-

Purification (Commentary)

Purification (Comment) Organism
prosubtilisin purified by ammonium sulfate precipitation and gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe 7-amido-4-methylcoumarin + H2O
-
 Bacillus subtilis ?
-
 ?
Ala-Ala-Phe 7-amido-4-methylcoumarin + H2O
-
 Bacillus subtilis JB1 ?
-
 ?
azocasein + H2O
-
 Bacillus subtilis ?
-
 ?
azocasein + H2O
-
 Bacillus subtilis JB1 ?
-
 ?
Bovine serum albumin + H2O
-
 Bacillus subtilis ?
-
 ?
Bovine serum albumin + H2O
-
 Bacillus subtilis JB1 ?
-
 ?
Fibrinogen + H2O
-
 Bacillus subtilis ?
-
 ?
Fibrinogen + H2O
-
 Bacillus subtilis JB1 ?
-
 ?
Gelatin + H2O
-
 Bacillus subtilis ?
-
 ?
Gelatin + H2O
-
 Bacillus subtilis JB1 ?
-
 ?

Synonyms

Synonyms Comment Organism
subtilisin JB1
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 recombinant prosubtilisin JB1, at 37°C Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
4 8 recombinant prosubtilisin JB1, at 37°C Bacillus subtilis