Cloned (Comment) | Organism |
---|---|
vector pET22b-AprB2 encoded residues 1-474 of the AprB2 precursor with a C-terminal His6 transformed into Escherichia coli DH5alpha. Expression construct transformed into Escherichia coli strain Rosetta-Gami(DE3)pLysS | Dichelobacter nodosus |
vector pET22b-AprV2 encoded residues 1-474 of the AprV2 precursor with a C-terminal His6 tag transformed into Escherichia coli strain Rosetta-Gami(DE3)pLysS | Dichelobacter nodosus |
vector pET22b-BprB encoded residues 1-476 of BprB with a C-terminal His6 tag transformed into Escherichia coli strain Rosetta-Gami(DE3)pLysS | Dichelobacter nodosus |
vector pET22b-BprV encoded residues 1-476 of BprV with a C-terminal His6 tag and transformed into Escherichia coli strain Rosetta-Gami(DE3)pLysS | Dichelobacter nodosus |
Crystallization (Comment) | Organism |
---|---|
by the hanging-drop vapour-diffusion method. Crystals of AprB2 diffract to 1.7 A resolution. The crystals belong to space group P1, with unit-cell parameters a = 42.7, b = 45.8, c = 45.7 A , alpha = 98.4, beta = 114.0, gamma = 114.6. The crystals contain one molecule in the asymmetric unit, with a solvent content of 36% | Dichelobacter nodosus |
by the hanging-drop vapour-diffusion method. Crystals of AprV2 diffract to 2.0 A resolution. The crystals belong to space group P1, with unit-cell parameters a = 43.1, b = 46.0, c = 47.2 A , alpha = 97.8, beta = 115.2, gamma = 115.2. The crystals contain one molecule in the asymmetric unit, with a solvent content of ca. 36% | Dichelobacter nodosus |
by the hanging-drop vapour-diffusion method. Crystals of BprB diffract to 1.8 A resolution. The crystals belong to space group P21, with unit-cell parameters a = 38.5, b = 90.5, c = 44.1 A , beta = 109.9. The crystals contain one molecule in the asymmetric unit, with a solvent content of 36% | Dichelobacter nodosus |
by the hanging-drop vapour-diffusion method. Crystals of BprV diffract to 2.0 A resolution. The crystals belong to space group P21, with unit-cell parameters a = 38.5, b = 89.6, c = 47.7 A , beta = 113.6. The crystals contain one molecule in the asymmetric unit, with a solvent content of 40% | Dichelobacter nodosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for catalysis | Dichelobacter nodosus | |
Ca2+ | is required for catalysis | Dichelobacter nodosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dichelobacter nodosus | - |
- |
- |
Dichelobacter nodosus | A5EVD0 | BprV | - |
Dichelobacter nodosus | A5EXI3 | AprV2 | - |
Dichelobacter nodosus | Q46547 | BprB | - |
Dichelobacter nodosus C305 | Q46547 | BprB | - |
Dichelobacter nodosus VCS1703A | A5EVD0 | BprV | - |
Dichelobacter nodosus VCS1703A | A5EXI3 | AprV2 | - |
Purification (Comment) | Organism |
---|---|
by affinity chromatography | Dichelobacter nodosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O | - |
Dichelobacter nodosus | N-methoxysuccinyl-Ala-Ala-Pro-Val + p-nitroaniline | - |
? | |
N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O | - |
Dichelobacter nodosus C305 | N-methoxysuccinyl-Ala-Ala-Pro-Val + p-nitroaniline | - |
? | |
N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O | - |
Dichelobacter nodosus VCS1703A | N-methoxysuccinyl-Ala-Ala-Pro-Val + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
subtilisin-like protease | - |
Dichelobacter nodosus |
General Information | Comment | Organism |
---|---|---|
physiological function | strains causing benign footrot secrete AprB2 | Dichelobacter nodosus |
physiological function | strains causing benign footrot secrete subtilisin-like protease BprB | Dichelobacter nodosus |
physiological function | strains causing virulent footrot secrete the subtilisin-like protease AprV2 | Dichelobacter nodosus |
physiological function | strains causing virulent footrot secrete the subtilisin-like protease BprV | Dichelobacter nodosus |