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Literature summary for 3.4.21.62 extracted from

  • Kazan, D.; Denizci, A.A.; Oner, M.N.; Erarslan, A.
    Purification and characterization of a serine alkaline protease from Bacillus clausii GMBAE 42 (2005), J. Ind. Microbiol. Biotechnol., 32, 335-344.
    View publication on PubMed
Search for more literature for 3.4.21.62

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: Tween-20, Tween-40, Tween-60, Tween-80 at 1%, sodium dodecylsulfate at 0.2%, for 1 h at 30°C. Not inhibitory: iodoacetate, ethylacetimide, phenylglyoxal, N-ethylmaleimide, N-bromosuccinimide Alkalihalobacillus clausii
phenylmethylsulfonyl fluoride 1 mM, 82% inhibition Alkalihalobacillus clausii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000655
-
 N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide 37°C, pH 10.5 Alkalihalobacillus clausii

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 5 mM, 40% increase in activity Alkalihalobacillus clausii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
 x * 26500, SDS-PAGE Alkalihalobacillus clausii

Organism

Organism UniProt Comment Textmining
Alkalihalobacillus clausii
-
 highest activity in late stationary growth phase in protein-rich medium at pH 10.5
-
Alkalihalobacillus clausii GMBAE 42
-
 highest activity in late stationary growth phase in protein-rich medium at pH 10.5
-

Purification (Commentary)

Purification (Comment) Organism
from culture filtrate Alkalihalobacillus clausii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6852
-
 37°C, pH 10.5 Alkalihalobacillus clausii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hammarsten casein + H2O activation energy for hydrolysis 10.59 kcal per mol Alkalihalobacillus clausii ?
-
 ?
Hammarsten casein + H2O activation energy for hydrolysis 10.59 kcal per mol Alkalihalobacillus clausii GMBAE 42 ?
-
 ?
N-succinyl-L-Ala-L-Ala-L-L-Ala-4-nitroanilide + H2O 3.8% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide Alkalihalobacillus clausii ?
-
 ?
N-succinyl-L-Ala-L-Ala-L-L-Ala-4-nitroanilide + H2O 3.8% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide Alkalihalobacillus clausii GMBAE 42 ?
-
 ?
N-succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O 19% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide Alkalihalobacillus clausii ?
-
 ?
N-succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O 19% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide Alkalihalobacillus clausii GMBAE 42 ?
-
 ?
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Alkalihalobacillus clausii ?
-
 ?
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Alkalihalobacillus clausii GMBAE 42 ?
-
 ?
N-succinyl-L-Ala-L-Ala-L-Val-4-nitroanilide + H2O 2.2% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide Alkalihalobacillus clausii ?
-
 ?
N-succinyl-L-Ala-L-Ala-L-Val-4-nitroanilide + H2O 2.2% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide Alkalihalobacillus clausii GMBAE 42 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 26500, SDS-PAGE Alkalihalobacillus clausii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 in absence of Ca2+ Alkalihalobacillus clausii
70
-
 in presence of 5 mM Ca2+ Alkalihalobacillus clausii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 pH 10.5, stable for 2 h Alkalihalobacillus clausii
50
-
 pH 10.5, 2 h, 85% residual activity Alkalihalobacillus clausii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
35.5
-
 Hammarsten casein 37°C, pH 10.5 Alkalihalobacillus clausii
70.2
-
 N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide 37°C, pH 10.5 Alkalihalobacillus clausii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11.3
-
-
 Alkalihalobacillus clausii

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 3 24 h, 76% loss of activity Alkalihalobacillus clausii
9 12.2 30°C, stable for 24 h Alkalihalobacillus clausii
12.7
-
 24 h, 38% loss of activity Alkalihalobacillus clausii