Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.62 extracted from

  • DeSantis, G.; Shang, X.; Jones, J.B.
    Toward tailoring of the S1 pocket of subtilisin B. lentus: Chemical modification of mutant enzymes as a strategy for removing specifiity limitations (1999), Biochemistry, 38, 13391-13397.
    View publication on PubMed
Search for more literature for 3.4.21.62

Protein Variants

Protein Variants Comment Organism
S166C site-directed mutagenesis combined with chemical modification Lederbergia lentus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C, mutant S166C-a Lederbergia lentus
0.7
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C, mutant S166C-f Lederbergia lentus
0.73
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
0.74
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C, mutant S166C-g Lederbergia lentus
0.8
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c Lederbergia lentus
1.09
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-e Lederbergia lentus
1.17
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c Lederbergia lentus
1.34
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-b Lederbergia lentus
1.5 2 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-h Lederbergia lentus
1.6
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
1.74
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-g Lederbergia lentus
1.9
-
 succinyl-AAPE-p-nitroanilide pH 8.6, 25°C mutant S166C-S-a Lederbergia lentus
1.9
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-e and S166S-f Lederbergia lentus
2
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
2.26
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-i Lederbergia lentus
2.46
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
3.4
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-b Lederbergia lentus
4.4
-
 succinyl-AAPE-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
5.2
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
5.3
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-i Lederbergia lentus
5.5
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
7.2
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
8.2
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-h Lederbergia lentus

Organism

Organism UniProt Comment Textmining
Lederbergia lentus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-AAPA-p-nitroanilide + H2O
-
 Lederbergia lentus ?
-
 ?
succinyl-AAPE-p-nitroanilide + H2O
-
 Lederbergia lentus ?
-
 ?
succinyl-AAPF-4-nitroanilide + H2O
-
 Lederbergia lentus succinyl-AAPF + 4-nitroaniline
-
 ?
succinyl-AAPR-p-nitroanilide + H2O
-
 Lederbergia lentus ?
-
 ?

Synonyms

Synonyms Comment Organism
EC 3.4.21.14 formerly included in Lederbergia lentus
SBL
-
 Lederbergia lentus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.052 2.1 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-g Lederbergia lentus
0.16
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
0.17
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-b Lederbergia lentus
0.35
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-h Lederbergia lentus
0.43
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-i Lederbergia lentus
0.68
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
1.06
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
1.75
-
 succinyl-AAPE-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
4.8
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-f Lederbergia lentus
6.08
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
6.08
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
6.8
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c and S166C-S-e Lederbergia lentus
9.65
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-g Lederbergia lentus
14.5
-
 succinyl-AAPE-p-nitroanilide pH 8.6, 25°C mutant S166C-S-a Lederbergia lentus
16.9
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c Lederbergia lentus
17.7
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
23.1
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c Lederbergia lentus
25
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-b Lederbergia lentus
28.2
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-f Lederbergia lentus
29
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-g Lederbergia lentus
47
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
48
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C, mutant S166C-h Lederbergia lentus
50
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-a Lederbergia lentus
67
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-i Lederbergia lentus
76
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
82
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-e Lederbergia lentus
153
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus