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Literature summary for 3.4.21.62 extracted from

  • Economou, C.; Chen, K.; Arnold, F.H.
    Random mutagenesis to enhance the activity of subtilisin in organic solvents: characterization of Q103R subtilisin E (1992), Biotechnol. Bioeng., 39, 658-662.
    View publication on PubMed
Search for more literature for 3.4.21.62

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
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 Q103R subtilisin E
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Bacillus subtilis
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 a clone producing Q103R subtilisin E
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Bacillus subtilis DB428
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 a clone producing Q103R subtilisin E
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
 Bacillus subtilis ?
-
 ?
Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
 Bacillus subtilis DB428 ?
-
 ?
additional information random mutagenesis to enhance the activity of subtilisin in organic solvents Bacillus subtilis ?
-
 ?
additional information random mutagenesis to enhance the activity of subtilisin in organic solvents Bacillus sp. (in: Bacteria) ?
-
 ?
additional information random mutagenesis to enhance the activity of subtilisin in organic solvents Bacillus subtilis DB428 ?
-
 ?
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
 Bacillus subtilis succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
 Bacillus subtilis DB428 succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bacillus subtilis
additional information
-
 additional information
-
 Bacillus sp. (in: Bacteria)