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Literature summary for 3.4.21.62 extracted from

  • Wells, J.A.; Estell, D.A.
    Subtilisin - an enzyme designed to be engineered (1988), Trends Biochem. Sci., 13, 291-297.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
almost every property of subtilisin has been altered by protein engineering, e.g. substrate specificity, pH-profile and stability to oxidative, thermal and alkaline inactivation Bacillus amyloliquefaciens

General Stability

General Stability Organism
Alteration of stability to oxidative, thermal and alkaline inactivation by protein engineering Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
expression of subtilisin BPN' gene isolated in a protease-deficient strain of Bacillus subtilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information alteration of substrate specificity by protein engineering Bacillus amyloliquefaciens ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
alteration of pH-profile by protein engineering Bacillus amyloliquefaciens