Literature summary for 3.4.21.61 extracted from

Schmidt, R.J.; Zhang, Y.; Zhao, Y.; Qian, Y.W.; Wang, H.; Lin, A.; Ehsani, M.E.; Yu, X.; Wang, G.; Singh, J.; Su, E.W.; Li, S.; Konrad, R.J.; Cao, G.
A novel splicing variant of proprotein convertase subtilisin/kexin type 9 (2008), DNA Cell Biol., 27, 183-189.

Search for more literature for 3.4.21.61

Cloned(Commentary)

Cloned (Comment)	Organism
cDNAs encoding full-length PCSK9 splicing variant or mutants inserted into a modified pJB02 vector with a C-terminal Flag-HIS6 or HIS6 tag, respectively. The resulting constructs used for transient transfection of HEK-293 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A443T	is expressed, processed, and secreted normally, and reduces cellular LDL uptake in a concentration-dependent like the wild-type	Homo sapiens
additional information	splicing variant of PCSK9 with an in-frame deletion of the eighth exon of 58 amino acids. Expressed in multiple tissues, including liver, small intestine, prostate, uterus, brain, and adipose tissue. Unlike wild-type PCSK9, which is secreted, the splicing variant expressed in HEK-293 cells fails to process the prosegment intracellularly and thus is not secreted into the medium. Splicing variant does not change the LDLR protein levels	Homo sapiens
N425S	is expressed, processed, and secreted normally, and reduces cellular LDL uptake in a concentration-dependent like the wild-type	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	splicing variant of PCSK9 has no specific effect impacting wild-type PCSK9 function	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64000	-	mature form of wild-type PCSK9, Western blot analysis	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
on Ni-NTA resin	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
intestine	-	Homo sapiens	-
liver	highest level of expression	Homo sapiens	-

Storage Stability

Storage Stability	Organism
-80°C	Homo sapiens

Synonyms

Synonyms	Comment	Organism
PCSK9	-	Homo sapiens
proprotein convertase subtilisin/kexin type 9	-	Homo sapiens

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Release 2023.1 (January 2023)