Literature summary for 3.4.21.61 extracted from

Liu, Z.X.; Fei, H.; Chi, C.W.
Two engineered eglin c mutants potently and selectively inhibiting kexin or furin (2004), FEBS Lett., 556, 116-120.

Search for more literature for 3.4.21.61

Cloned(Commentary)

Cloned (Comment)	Organism
cloned and expressed in Pichia pastoris	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
eglin c mutant D42R	-	Saccharomyces cerevisiae
eglin c mutant L45R	-	Saccharomyces cerevisiae
eglin c mutant Tyr replaced with Glu at P4	-	Saccharomyces cerevisiae
eglin variant M1 RVTR	-	Saccharomyces cerevisiae
eglin variant M2 RVKR	-	Saccharomyces cerevisiae
eglin variant M3 RVTRDERY	-	Saccharomyces cerevisiae
eglin variant M4 RVTRDRRY	-	Saccharomyces cerevisiae
eglin variant M5 RVTRDLDY	-	Saccharomyces cerevisiae
eglin variant M6 RVTRDLRR	-	Saccharomyces cerevisiae
eglin variant M7 RVTRDLRE	-	Saccharomyces cerevisiae
eglin variant M8 RVTRDARY	-	Saccharomyces cerevisiae
additional information	no inhibition with eglin c mutant Arg replaced with Asp at P3	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	trans Golgi network	Saccharomyces cerevisiae	5794	-

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	yeast	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00000003	-	eglin c mutant Tyr replaced with Glu at P4	pH 7.5, 37°C	Saccharomyces cerevisiae
0.000000038	-	eglin variant M2 RVKR	pH 7.5, 37°C	Saccharomyces cerevisiae
0.000000128	-	eglin variant M1 RVTR	pH 7.5, 37°C	Saccharomyces cerevisiae
0.00000013	-	eglin c mutant L45R	pH 7.5, 37°C	Saccharomyces cerevisiae
0.00000013	-	eglin c mutant D42R	pH 7.5, 37°C	Saccharomyces cerevisiae
0.000000136	-	eglin variant M8 RVTRDARY	pH 7.5, 37°C	Saccharomyces cerevisiae
0.000000316	-	eglin variant M3 RVTRDERY	pH 7.5, 37°C	Saccharomyces cerevisiae
0.0000043	-	eglin variant M5 RVTRDLDY	pH 7.5, 37°C	Saccharomyces cerevisiae
0.0000267	-	eglin variant M6 RVTRDLRR	pH 7.5, 37°C	Saccharomyces cerevisiae
0.000039	-	eglin variant M7 RVTRDLRE	pH 7.5, 37°C	Saccharomyces cerevisiae
0.000089	-	eglin variant M4 RVTRDRRY	pH 7.5, 37°C	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)