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Literature summary for 3.4.21.61 extracted from

  • Rockwell, N.C.; Fuller, R.S.
    Interplay between S1 and S4 subsites in Kex2 protease: Kex2 exhibits dual specificity for the P4 side chain (1998), Biochemistry, 37, 3386-3391.
    View publication on PubMed
Search for more literature for 3.4.21.61

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 Ac-Nle-YKR 4-methylcoumarin 7-amide pH 7.0, 21°C Saccharomyces cerevisiae
0.038
-
 Ac-Nle-YKK 4-methylcoumarin 7-amide pH 7.0, 21°C Saccharomyces cerevisiae
0.32
-
 t-butyloxycarbonyl-QGR 4-methylcoumarin 7-amide pH 7.0, 21°C Saccharomyces cerevisiae
0.35
-
 Ac-AYKK 4-methylcoumarin 7-amide pH 7.0, 21°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 yeast, strain X-2180, haploid cells
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-(beta-cyclohexyl)alanineYKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-(beta-cyclohexyl)alanineYKK
-
 ?
Ac-alpha-aminobutyric acid-YKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-alpha-aminobutyric acid-YKK
-
 ?
Ac-Arg-Tyr-Lys-Lys 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-Arg-Tyr-Lys-Lys
-
 ?
Ac-AYKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-AYKK
-
 ?
Ac-AYKR 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-AYKR
-
 ?
Ac-Cit-Tyr-Lys-Lys 4-methylcoumarin 7-amide + H2O this substrate is cleaved poorly Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-Cit-Tyr-Lys-Lys 4-methylcoumarin
-
 ?
Ac-CYKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-CYKK
-
 ?
Ac-FYKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-FYKK
-
 ?
Ac-Nle-YKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-Nle-YKK
-
 ?
Ac-Nle-YKR 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-Nle-YKR
-
 ?
Ac-norvaline-YKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-norvaline-YKK
-
 ?
Ac-Pro-Met-Tyr-Lys-Arg 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-Pro-Met-Tyr-Lys-Arg
-
 ?
Ac-RYKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-RYKK
-
 ?
Ac-VYKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-VYKK
-
 ?
additional information exhibits optimal activity toward substrates with Lys or Arg at P2 and Arg at P1, also recognizes P4, with dual specificity for aliphatic and basic residues Saccharomyces cerevisiae ?
-
 ?
t-butyloxycarbonyl-EKK 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + t-butyloxycarbonyl-EKK
-
 ?
t-butyloxycarbonyl-QGR 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + t-butyloxycarbonyl-QGR
-
 ?

Synonyms

Synonyms Comment Organism
proprotein processing protease
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.4
-
 t-butyloxycarbonyl-EKK 4-methylcoumarin 7-amide pH 7.0, 21°C Saccharomyces cerevisiae
21.6
-
 Ac-AYKK 4-methylcoumarin 7-amide pH 7.0, 21°C Saccharomyces cerevisiae
300
-
 Ac-Nle-YKK 4-methylcoumarin 7-amide pH 7.0, 21°C Saccharomyces cerevisiae
366
-
 t-butyloxycarbonyl-QGR 4-methylcoumarin 7-amide pH 7.0, 21°C Saccharomyces cerevisiae
3000
-
 Ac-Nle-YKR 4-methylcoumarin 7-amide pH 7.0, 21°C Saccharomyces cerevisiae