Protein Variants | Comment | Organism |
---|---|---|
I16L | mutation disrupts activation process of the factor X zymogen with alteration of active site function and impairment of Na+ and factor Va binding. Irreversible stabilization with L-Glu-Gly-L-Arg-chloromethyl ketone fully rescues factor Va binding. The Km value for prothrombin conversion for the mutant assembled into prothrombinase is unaltered, whereas the kcat is reduced 3- to 4fold | Homo sapiens |
V17A | mutation disrupts activation process of the factor X zymogen with alteration of active site function and impairment of Na+ and factor Va binding. The Km value for prothrombin conversion for the mutant assembled into prothrombinase is unaltered, whereas the kcat is reduced 3- to 4fold | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pefabloc | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00031 | - |
prothrombin | prothrombinase assembled with mutant I16L, pH 7.5 | Homo sapiens | |
0.00035 | - |
prothrombin | prothrombinase assembled with recombinant factor Xa, pH 7.5 | Homo sapiens | |
0.00042 | - |
prothrombin | prothrombinase assembled with native factor Xa, pH 7.5 | Homo sapiens | |
0.00047 | - |
prothrombin | prothrombinase assembled with mutant V17A, pH 7.5 | Homo sapiens | |
0.0052 | - |
prethrombin-1 | prothrombinase assembled with recombinant factor Xa, pH 7.5 | Homo sapiens | |
0.0054 | - |
prethrombin-1 | prothrombinase assembled with native factor Xa, pH 7.5 | Homo sapiens | |
0.0057 | - |
prethrombin-1 | prothrombinase assembled with mutant I16L, pH 7.5 | Homo sapiens | |
0.0069 | - |
prethrombin-1 | prothrombinase assembled with mutant V17A, pH 7.5 | Homo sapiens | |
0.072 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | native factor Xa, pH 7.5 | Homo sapiens | |
0.089 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | recombinant factor Xa, pH 7.5 | Homo sapiens | |
0.13 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | prothrombinase assembled with recombinant factor Xa, pH 7.5 | Homo sapiens | |
0.17 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | prothrombinase assembled with native factor Xa, pH 7.5 | Homo sapiens | |
0.29 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | prothrombinase assembled with mutant I16L, pH 7.5 | Homo sapiens | |
0.49 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | prothrombinase assembled with mutant V17A, pH 7.5 | Homo sapiens | |
1.15 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | mutant I16L, pH 7.5 | Homo sapiens | |
1.38 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | mutant V17A, pH 7.5 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide + H2O | - |
Homo sapiens | methoxycarbonylcyclohexyl-Gly-Gly-L-Arg + 4-nitroaniline | - |
? | |
prethrombin-1 + H2O | - |
Homo sapiens | thrombin + ? | - |
? | |
prothrombin + H2O | - |
Homo sapiens | thrombin + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
prethrombin-1 | prothrombinase assembled with mutant V17A, pH 7.5 | Homo sapiens | |
6.6 | - |
prethrombin-1 | prothrombinase assembled with mutant I16L, pH 7.5 | Homo sapiens | |
10.3 | - |
prothrombin | prothrombinase assembled with mutant I16L, pH 7.5 | Homo sapiens | |
14.8 | - |
prothrombin | prothrombinase assembled with mutant V17A, pH 7.5 | Homo sapiens | |
17.5 | - |
prethrombin-1 | prothrombinase assembled with recombinant factor Xa, pH 7.5 | Homo sapiens | |
18.1 | - |
prethrombin-1 | prothrombinase assembled with native factor Xa, pH 7.5 | Homo sapiens | |
32.3 | - |
prothrombin | prothrombinase assembled with recombinant factor Xa, pH 7.5 | Homo sapiens | |
33 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | prothrombinase assembled with mutant I16L, pH 7.5 | Homo sapiens | |
40.4 | - |
prothrombin | prothrombinase assembled with native factor Xa, pH 7.5 | Homo sapiens | |
58 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | mutant I16L, pH 7.5 | Homo sapiens | |
72 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | prothrombinase assembled with native factor Xa, pH 7.5 | Homo sapiens | |
73 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | mutant V17A, pH 7.5 | Homo sapiens | |
91 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | prothrombinase assembled with mutant V17A, pH 7.5 | Homo sapiens | |
140 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | native factor Xa, pH 7.5 | Homo sapiens | |
140 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | prothrombinase assembled with recombinant factor Xa, pH 7.5 | Homo sapiens | |
210 | - |
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide | recombinant factor Xa, pH 7.5 | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
pefabloc | prothrombinase assembled with recombinant factor Xa, pH 7.5 | Homo sapiens | |
0.000053 | - |
pefabloc | prothrombinase assembled with native factor Xa, pH 7.5 | Homo sapiens | |
0.000064 | - |
pefabloc | native factor Xa, pH 7.5 | Homo sapiens | |
0.00007 | - |
pefabloc | recombinant factor Xa, pH 7.5 | Homo sapiens | |
0.00021 | - |
pefabloc | prothrombinase assembled with mutant I16L, pH 7.5 | Homo sapiens | |
0.00029 | - |
pefabloc | prothrombinase assembled with mutant V17A, pH 7.5 | Homo sapiens | |
0.00169 | - |
pefabloc | mutant V17A, pH 7.5 | Homo sapiens | |
0.0017 | - |
pefabloc | mutant I16L, pH 7.5 | Homo sapiens |