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Literature summary for 3.4.21.6 extracted from

  • Yang, L.; Manithody, C.; Qureshi, S.H.; Rezaie, A.R.
    Factor Va alters the conformation of the Na+-binding loop of factor Xa in the prothrombinase complex (2008), Biochemistry, 47, 5976-5985.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.6

Activating Compound

Activating Compound Comment Organism Structure
Factor Va the Na+ and factor Va binding sites are energetically linked. A cofactor function for factor Va in the prothrombinase complex involves inducing a conformational change in the 220-loop of factor Xa that appears to stabilize this loop in the Na+-bound conformation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
E217A affinity for interaction with Na+ is impaired. 3-4fold decrease in activity towards both natural and synthetic substrates. Presence of factor Va restores most of the catalytic defect with substrate prothrombin Homo sapiens
E217Q affinity for interaction with Na+ is impaired. Presence of factor Va restores most of the catalytic defect with substrate prothrombin Homo sapiens
L224A affinity for interaction with Na+ is impaired Homo sapiens
R221A affinity for interaction with Na+ is impaired Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0507
-
 Arg-Gly-Arg-4-nitroanilide mutant K222A, pH 7.5 Homo sapiens
0.0517
-
 Arg-Gly-Arg-4-nitroanilide wild-type, pH 7.5 Homo sapiens
0.191
-
 Arg-Gly-Arg-4-nitroanilide mutant R221A, pH 7.5 Homo sapiens
0.95
-
 Arg-Gly-Arg-4-nitroanilide mutant E217Q, pH 7.5, presence of 0.5 M NaCl Homo sapiens
2
-
 Arg-Gly-Arg-4-nitroanilide mutant E217A, pH 7.5, presence of 0.5 M NaCl Homo sapiens
2.05
-
 Arg-Gly-Arg-4-nitroanilide mutant E217Q, pH 7.5 Homo sapiens
3.9
-
 Arg-Gly-Arg-4-nitroanilide mutant E217A, pH 7.5 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ ionic interaction between residues E217 and K224 stabilizes the 220-loop of factor Xa for binding Na+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Gly-Arg-4-nitroanilide + H2O
-
 Homo sapiens Arg-Gly-Arg + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27.1
-
 Arg-Gly-Arg-4-nitroanilide mutant E217A, pH 7.5 Homo sapiens
57.2
-
 Arg-Gly-Arg-4-nitroanilide mutant E217A, pH 7.5, presence of 0.5 M NaCl Homo sapiens
94.3
-
 Arg-Gly-Arg-4-nitroanilide mutant E217Q, pH 7.5 Homo sapiens
99.2
-
 Arg-Gly-Arg-4-nitroanilide mutant K222A, pH 7.5 Homo sapiens
125.3
-
 Arg-Gly-Arg-4-nitroanilide wild-type, pH 7.5 Homo sapiens
151.7
-
 Arg-Gly-Arg-4-nitroanilide mutant E217Q, pH 7.5, presence of 0.5 M NaCl Homo sapiens
154.4
-
 Arg-Gly-Arg-4-nitroanilide mutant R221A, pH 7.5 Homo sapiens