Protein Variants | Comment | Organism |
---|---|---|
S195A | catalytically inactive mutant, that still binds to inhibitor antithrombin with the same velocity as the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antithrombin | human recombinant protein, competitive inhibition, heparin-activated antithrombin, interaction and inhibition mechanism involving inhibitor residues Tyr253, as well as Glu255, Glu237, and Arg399, and exosites generated via heparin binding, rapid kinetic studies, wild-type and mutant antithrombins, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | rapid inhibition kinetic studies, wild-type and mutant antithrombins, overview | Homo sapiens |