Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-dicaproyl-sn-glycero-3-phospho-L-serine | i.e. C6PS, soluble phospholipid, inhibition of amidolytic activity of factor Xa in absence of Ca2+ | Bos taurus | |
1,2-dioleoyl-phosphatidylglycerol | in vesicles with 1,2-dioleoyl-phosphatidylglycerol, 18fold activation of enzyme activity with prothrombin | Bos taurus | |
1-palmitoyl-2-oleoyl-3-sn-phosphatidylcholine | in vesicles with 1,2-palmitoyl-2-oleoyl-3-sn-phosphatidylcholine, 18fold activation of enzyme activity with prothrombin | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, SDS-PAGE | Bos taurus |
47000 | - |
enzyme in absence of Ca2+ and 1,2-dicaproyl-sn-glycero-3-phospho-L-serine, native PAGE | Bos taurus |
114000 | - |
enzyme in presence of 5 mM Ca2+ and 0.4 mM 1,2-dicaproyl-sn-glycero-3-phospho-L-serine, native PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prothrombin + H2O | Bos taurus | crucial step in blood coagulation process | thrombin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | processing and activation of the recombinant wild-type and mutant zymogens to mature enzymes by Russell's viper venom | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin | scutelarin has similar specificity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide + H2O | i.e. S-2765, synthetic chromogenic substrate | Bos taurus | N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine + 4-nitroaniline | - |
? | |
prothrombin + H2O | - |
Bos taurus | thrombin + ? | - |
? | |
prothrombin + H2O | crucial step in blood coagulation process | Bos taurus | thrombin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Bos taurus |
More | factor Xa is part of the prothrombinase complex with Ca2+, phospholipids and factor Va, binding of 1,2-dicaproyl-sn-glycero-3-phospho-L-serine to the second binding site in presence of Ca2+ leads to dimerization | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
factor Xa | - |
Bos taurus |
More | the enzyme belongs to the peptidase family S1, i.e. trypsin family | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
1,2-dicaproyl-sn-glycero-3-phospho-L-serine | i.e. C6PS, soluble phospholipid, 2 molecules per enzyme molecule, binding to 2 binding sites in sigmoidal fashion with active site labeled enzyme and in hyperbolic fashion with nonlabeled enzyme, binding induces conformaional changes of secondary structure, binding to the tighter of the 2 binding site triggers structural changes which lead to Ca2+-dependent dimer formation, model of sequential binding mechanism | Bos taurus | |
Factor Va | - |
Bos taurus |