Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.6 extracted from

  • Majumder, R.; Wang, J.; Lentz, B.R.
    Effects of water-soluble phosphatidylserine on bovine factor Xa: functional and structural changes plus dimerization (2003), Biophys. J., 84, 1238-1251.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1,2-dicaproyl-sn-glycero-3-phospho-L-serine i.e. C6PS, soluble phospholipid, inhibition of amidolytic activity of factor Xa in absence of Ca2+ Bos taurus
1,2-dioleoyl-phosphatidylglycerol in vesicles with 1,2-dioleoyl-phosphatidylglycerol, 18fold activation of enzyme activity with prothrombin Bos taurus
1-palmitoyl-2-oleoyl-3-sn-phosphatidylcholine in vesicles with 1,2-palmitoyl-2-oleoyl-3-sn-phosphatidylcholine, 18fold activation of enzyme activity with prothrombin Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
1 * 45000, SDS-PAGE Bos taurus
47000
-
enzyme in absence of Ca2+ and 1,2-dicaproyl-sn-glycero-3-phospho-L-serine, native PAGE Bos taurus
114000
-
enzyme in presence of 5 mM Ca2+ and 0.4 mM 1,2-dicaproyl-sn-glycero-3-phospho-L-serine, native PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prothrombin + H2O Bos taurus crucial step in blood coagulation process thrombin + ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification processing and activation of the recombinant wild-type and mutant zymogens to mature enzymes by Russell's viper venom Bos taurus

Reaction

Reaction Comment Organism Reaction ID
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide + H2O i.e. S-2765, synthetic chromogenic substrate Bos taurus N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine + 4-nitroaniline
-
?
prothrombin + H2O
-
Bos taurus thrombin + ?
-
?
prothrombin + H2O crucial step in blood coagulation process Bos taurus thrombin + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Bos taurus
More factor Xa is part of the prothrombinase complex with Ca2+, phospholipids and factor Va, binding of 1,2-dicaproyl-sn-glycero-3-phospho-L-serine to the second binding site in presence of Ca2+ leads to dimerization Bos taurus

Synonyms

Synonyms Comment Organism
factor Xa
-
Bos taurus
More the enzyme belongs to the peptidase family S1, i.e. trypsin family Bos taurus

Cofactor

Cofactor Comment Organism Structure
1,2-dicaproyl-sn-glycero-3-phospho-L-serine i.e. C6PS, soluble phospholipid, 2 molecules per enzyme molecule, binding to 2 binding sites in sigmoidal fashion with active site labeled enzyme and in hyperbolic fashion with nonlabeled enzyme, binding induces conformaional changes of secondary structure, binding to the tighter of the 2 binding site triggers structural changes which lead to Ca2+-dependent dimer formation, model of sequential binding mechanism Bos taurus
Factor Va
-
Bos taurus