Application | Comment | Organism |
---|---|---|
medicine | viruses utilizes the enzyme to trigger their infectivity and multiplication in lungs, an inhibitor may regulate the enzyme activity and viral infections in vivo | Sus scrofa |
Crystallization (Comment) | Organism |
---|---|
- |
Sus scrofa |
General Stability | Organism |
---|---|
heparin stabilizes the enzyme, retains about 50% activity in a low-salt solution after 2 h incubation at 25°C | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Sus scrofa | |
Aprotinin | - |
Sus scrofa | |
benzamidine | - |
Sus scrofa | |
diisopropylfluorophosphate | - |
Sus scrofa | |
leupeptin | - |
Sus scrofa | |
additional information | chymostatin, E64 and pestatin have no effect | Sus scrofa | |
mucus protease inhibitor | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mast cell | - |
Sus scrofa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Sus scrofa |
120000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sus scrofa | post-translational proteolytic cleavage of the precursors of the fusion glycoproteins of enveloped RNA viruses is indispensable for their fusion activity and infectivity, enzyme triggers infection by pneumotropic Sendai and influenza A viruses by processing | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
pig | - |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3930 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-Ser-Ile-Gln-Ser-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 51.2% | Sus scrofa | ? | - |
? | |
human influenza virus HA + H2O | - |
Sus scrofa | HA1 + HA2 | - |
? | |
additional information | activity only toward substrates with Arg at the P1 position, only 0.3% and 0.1% activity with t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide and Arg-4-methylcoumarin 7-amide, Suc-Ala-Pro-Ala-4-methylcoumarin 7-amide and Suc-Leu-Leu-Val-Leu-4-methylcoumarin 7-amide are no substrates | Sus scrofa | ? | - |
? | |
additional information | post-translational proteolytic cleavage of the precursors of the fusion glycoproteins of enveloped RNA viruses is indispensable for their fusion activity and infectivity, enzyme triggers infection by pneumotropic Sendai and influenza A viruses by processing | Sus scrofa | ? | - |
? | |
Pro-Phe-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 3.9% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Gln-Ala-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 100% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Gln-Arg-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 26.0% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Gln-Gly-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 63.3% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Glu(OBzl)-Ala-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 67.3% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Glu(OBzl)-Gly-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 26.3% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Glu-Lys-Lys-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 1.6% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Gly-Lys-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 10.9% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Ile-Glu-Gly-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 3.5% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Leu-Arg-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 5.9% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Leu-Gly-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 30.0% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Leu-Lys-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 14.4% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Phe-Ser-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 36.3% | Sus scrofa | ? | - |
? | |
t-butyloxycarbonyl-Val-Pro-Arg-4-methyl-coumarin 7-amide + H2O | synthetic substrate, relative activity 6.1% | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
Mast cell tryptase | - |
Sus scrofa |
tryptase | - |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
no activity below | Sus scrofa |