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Literature summary for 3.4.21.59 extracted from

  • Harvima, I.T.; Schechter, N.M.; Harvima, R.J.; Fräki, J.E.
    Human skin tryptase: purification, partial characterization and comparison with human lung tryptase (1988), Biochim. Biophys. Acta, 957, 71-80.
    View publication on PubMed

General Stability

General Stability Organism
addition of protein to the tryptase-heparin mixture is needed to keep the enzyme stable Homo sapiens
heparin glycosaminoglycan partially stabilizes the tryptase Homo sapiens
In low-salt buffer the enzyme is labile, at least 1.4 M KCl is needed to keep the enzyme stable if incubated at 37°C for 30 min Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
benzamidine
-
Homo sapiens
diisopropyl fluorophosphate
-
Homo sapiens
leupeptin
-
Homo sapiens
N-tosyl-L-Phe chloromethyl ketone
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000 + x * 38000, human, SDS-PAGE Homo sapiens
38000
-
x * 34000 + x * 38000, human, SDS-PAGE Homo sapiens
120000
-
human, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
Homo sapiens
-
skin
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzyloxycarbonyl-Arg 4-nitroanilide + H2O
-
Homo sapiens Benzyloxycarbonyl-Arg + 4-nitroaniline
-
?
Benzyloxycarbonyl-Gly-Arg 4-trifluoromethylcoumarin 7-amide + H2O
-
Homo sapiens Benzyloxycarbonyl-Gly-Arg + 7-amino-4-trifluoromethylcoumarin
-
?
Benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide + H2O
-
Homo sapiens Benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline
-
?
Benzyloxycarbonyl-Lys-Arg 4-trifluoromethylcoumarin 7-amide + H2O
-
Homo sapiens Benzyloxycarbonyl-Lys-Arg + 7-amino-4-trifluoromethylcoumarin
-
?

Subunits

Subunits Comment Organism
tetramer x * 34000 + x * 38000, human, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.35
-
benzyloxycarbonyl-Arg 4-nitroanilide skin enzyme Homo sapiens
5.52
-
benzyloxycarbonyl-Arg 4-nitroanilide lung enzyme Homo sapiens
45.7
-
Benzyloxycarbonyl-Lys-Arg 4-trifluoromethylcoumarin 7-amide skin and lung enzyme Homo sapiens
104
-
Benzyloxycarbonyl-Gly-Arg 4-trifluoromethylcoumarin 7-amide skin enzyme Homo sapiens
107
-
Benzyloxycarbonyl-Gly-Arg 4-trifluoromethylcoumarin 7-amide lung enzyme Homo sapiens
551
-
Benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide skin enzyme Homo sapiens
553
-
Benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide lung enzyme Homo sapiens