Literature summary for 3.4.21.57 extracted from

  • Kim, J.I.; Lee, S.M.; Jung, H.J.
    Characterization of calcium-activated bifunctional peptidase of the psychrotrophic Bacillus cereus (2005), J. Microbiol., 43, 237-243.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 20% inhibition at 0.01 mM, 55% inhibition at 0.1 mM, 64% inhibition at 0.5 mM Bacillus cereus
bestatin 6% inhibition at 0.001 mM, 39% inhibition at 0.005 mM, 54% inhibition at 0.01 mM, 57% inhibition at 0.1 mM Bacillus cereus
Cu2+ 87% inhibition at 1 mM Bacillus cereus
EDTA 45% inhibition at 0.01 mM, 64% inhibition at 0.1 mM, 84% inhibition at 0.5 mM Bacillus cereus
EGTA 17% inhibition at 0.01 mM, 44% inhibition at 0.1 mM, 44% inhibition at 0.5 mM Bacillus cereus
Fe2+ 100% inhibition at 0.1 mM Bacillus cereus
phenylmethylsulfonyl fluoride 67% inhibition at 0.01 mM, 100% inhibition above 0.1 mM Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.315
-
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide 50 mM Tris-HCl pH 8.0, 37°C, 10 min Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 39% activation at 1 mM Bacillus cereus
Mg2+ 56% activation at 1 mM Bacillus cereus
Ca2+ 86% activation at 1 mM Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Bacillus cereus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Bacillus cereus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
-
Bacillus cereus N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
Leucine endopeptidase
-
Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bacillus cereus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70 30% of activity at 10°C and 70°C Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
complete loss of acivity after 30 min at 70°C Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
Bacillus cereus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0067
-
bestatin 50 mM Tris-HCl pH 8.0, 37°C, 10 min Bacillus cereus