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Literature summary for 3.4.21.55 extracted from

  • Pirkle, H.; Theodor, I.; Miyada, D.; Simmons, G.
    Thrombin-like enzyme from the venom of Bitis gabonica. Purification, properties, and coagulant actions (1986), J. Biol. Chem., 261, 8830-8835.
    View publication on PubMed

General Stability

General Stability Organism
requires Ca2+ for stability in crude venom, during purification and storage of purified enzyme Bitis gabonica

Inhibitors

Inhibitors Comment Organism Structure
fibrinogen substrate inhibition, 3 mg/ml and above Bitis gabonica
additional information heparin or hirudin (with or without antithrombin III) Bitis gabonica
PMSF
-
Bitis gabonica
tosyl-L-lysine chloromethyl ketone
-
Bitis gabonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
tosyl-L-arginine methyl ester
-
Bitis gabonica
0.82
-
H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide
-
Bitis gabonica
0.13
-
Tosyl-Gly-Pro-Arg 4-nitroanilide
-
Bitis gabonica
3.4
-
H-D-Phenylglycine-Phe-Arg 4-nitroanilide
-
Bitis gabonica
0.88
-
H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide
-
Bitis gabonica
2.72
-
tosyl-Gly-Pro-Lys 4-nitroanilide
-
Bitis gabonica

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Bitis gabonica
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition Bitis gabonica
additional information
-
compared to that of related enzymes Bitis gabonica
additional information
-
N-terminal sequence homology to crotolase and B-chain of thrombin Bitis gabonica
30600
-
1 * 30600, Bitis gabonica, SDS-PAGE, reducing and nonreducing conditions Bitis gabonica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fibrinogen + H2O Bitis gabonica clots fibrinogen with 45 NIH (National Institute of Health) thrombin equivalent units/mg ?
-
?

Organism

Organism UniProt Comment Textmining
Bitis gabonica
-
Gaboon viper
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content: 20.6% Bitis gabonica
glycoprotein Asn-linked complex-type oligosaccharides Bitis gabonica

Purification (Commentary)

Purification (Comment) Organism
-
Bitis gabonica

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Bitis gabonica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
44.9, equivalent to NIH (National Institute of Health)-units of thrombin Bitis gabonica

Storage Stability

Storage Stability Organism
4°C, in the presence of Ca2+, 2 years Bitis gabonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information insulin, glucagon, S-peptide of ribonuclease, H-D-cyclohexylglycyl-alpha-aminobutyryl-Arg 4-nitroanilide, benzoyl-Pro-Phe-Arg 4-nitroanilide, H-D-2-aminobutyryl-cyclohexylalanyl-Lys 4-nitroanilide, H-D-Nle-hexahydrotyrosyl-Lys 4-nitroanilide, benzyloxycarbonyl-gamma (alpha-t-butoxy)glutamyl-Gly-Arg 4-nitroanilide, methoxycarbonyl-D-cyclohexylglycyl-Arg 4-nitroanilide Bitis gabonica ?
-
?
Fibrinogen + H2O removes fibrinopeptide A and than B from fibrinogen Bitis gabonica Fibrinopeptide A + fibrinopeptide B + ?
-
?
Human blood coagulation factor XIII + H2O activates factor XIII Bitis gabonica Activated human blood coagulation factor XIII
-
?
Tosyl-L-Arg methyl ester + H2O i.e. Nalpha-p-tosyl-L-arginine methyl ester Bitis gabonica ?
-
?
Tripeptide nitroanilide derivatives + H2O poor substrates Bitis gabonica Tripeptide + 4-nitroaniline
-
?
Tosyl-Gly-Pro-Arg 4-nitroanilide + H2O
-
Bitis gabonica ?
-
?
H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide + H2O
-
Bitis gabonica ?
-
?
H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide + H2O
-
Bitis gabonica ?
-
?
Tosyl-Gly-Pro-Lys 4-nitroanilide + H2O
-
Bitis gabonica ?
-
?
H-D-phenylglycine-Phe-Arg 4-nitroanilide + H2O
-
Bitis gabonica ?
-
?
Fibrinogen + H2O clots fibrinogen with 45 NIH (National Institute of Health) thrombin equivalent units/mg Bitis gabonica ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30600, Bitis gabonica, SDS-PAGE, reducing and nonreducing conditions Bitis gabonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at, natural substrates Bitis gabonica
30
-
assay at, tosyl-Arg-methyl ester as substrate Bitis gabonica
27
-
assay at Bitis gabonica
27
-
synthetic tripeptide amide substrates Bitis gabonica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
Tosyl-Gly-Pro-Arg 4-nitroanilide
-
Bitis gabonica
0.065
-
H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide
-
Bitis gabonica
0.204
-
tosyl-Gly-Pro-Lys 4-nitroanilide
-
Bitis gabonica
0.248
-
H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide
-
Bitis gabonica
0.952
-
H-D-Phenylglycine-Phe-Arg 4-nitroanilide
-
Bitis gabonica
12.2
-
tosyl-L-Arg methyl ester
-
Bitis gabonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 5.3 Bitis gabonica