General Stability | Organism |
---|---|
requires Ca2+ for stability in crude venom, during purification and storage of purified enzyme | Bitis gabonica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fibrinogen | substrate inhibition, 3 mg/ml and above | Bitis gabonica | |
additional information | heparin or hirudin (with or without antithrombin III) | Bitis gabonica | |
PMSF | - |
Bitis gabonica | |
tosyl-L-lysine chloromethyl ketone | - |
Bitis gabonica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
tosyl-L-arginine methyl ester | - |
Bitis gabonica | |
0.13 | - |
Tosyl-Gly-Pro-Arg 4-nitroanilide | - |
Bitis gabonica | |
0.82 | - |
H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide | - |
Bitis gabonica | |
0.88 | - |
H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide | - |
Bitis gabonica | |
2.72 | - |
tosyl-Gly-Pro-Lys 4-nitroanilide | - |
Bitis gabonica | |
3.4 | - |
H-D-Phenylglycine-Phe-Arg 4-nitroanilide | - |
Bitis gabonica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bitis gabonica | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Bitis gabonica |
additional information | - |
compared to that of related enzymes | Bitis gabonica |
additional information | - |
N-terminal sequence homology to crotolase and B-chain of thrombin | Bitis gabonica |
30600 | - |
1 * 30600, Bitis gabonica, SDS-PAGE, reducing and nonreducing conditions | Bitis gabonica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | Bitis gabonica | clots fibrinogen with 45 NIH (National Institute of Health) thrombin equivalent units/mg | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bitis gabonica | - |
Gaboon viper | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content: 20.6% | Bitis gabonica |
glycoprotein | Asn-linked complex-type oligosaccharides | Bitis gabonica |
Purification (Comment) | Organism |
---|---|
- |
Bitis gabonica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bitis gabonica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
44.9, equivalent to NIH (National Institute of Health)-units of thrombin | Bitis gabonica |
Storage Stability | Organism |
---|---|
4°C, in the presence of Ca2+, 2 years | Bitis gabonica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | removes fibrinopeptide A and than B from fibrinogen | Bitis gabonica | Fibrinopeptide A + fibrinopeptide B + ? | - |
? | |
Fibrinogen + H2O | clots fibrinogen with 45 NIH (National Institute of Health) thrombin equivalent units/mg | Bitis gabonica | ? | - |
? | |
H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide + H2O | - |
Bitis gabonica | ? | - |
? | |
H-D-phenylglycine-Phe-Arg 4-nitroanilide + H2O | - |
Bitis gabonica | ? | - |
? | |
H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide + H2O | - |
Bitis gabonica | ? | - |
? | |
Human blood coagulation factor XIII + H2O | activates factor XIII | Bitis gabonica | Activated human blood coagulation factor XIII | - |
? | |
additional information | insulin, glucagon, S-peptide of ribonuclease, H-D-cyclohexylglycyl-alpha-aminobutyryl-Arg 4-nitroanilide, benzoyl-Pro-Phe-Arg 4-nitroanilide, H-D-2-aminobutyryl-cyclohexylalanyl-Lys 4-nitroanilide, H-D-Nle-hexahydrotyrosyl-Lys 4-nitroanilide, benzyloxycarbonyl-gamma (alpha-t-butoxy)glutamyl-Gly-Arg 4-nitroanilide, methoxycarbonyl-D-cyclohexylglycyl-Arg 4-nitroanilide | Bitis gabonica | ? | - |
? | |
Tosyl-Gly-Pro-Arg 4-nitroanilide + H2O | - |
Bitis gabonica | ? | - |
? | |
Tosyl-Gly-Pro-Lys 4-nitroanilide + H2O | - |
Bitis gabonica | ? | - |
? | |
Tosyl-L-Arg methyl ester + H2O | i.e. Nalpha-p-tosyl-L-arginine methyl ester | Bitis gabonica | ? | - |
? | |
Tripeptide nitroanilide derivatives + H2O | poor substrates | Bitis gabonica | Tripeptide + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30600, Bitis gabonica, SDS-PAGE, reducing and nonreducing conditions | Bitis gabonica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Bitis gabonica |
27 | - |
synthetic tripeptide amide substrates | Bitis gabonica |
30 | - |
assay at, tosyl-Arg-methyl ester as substrate | Bitis gabonica |
37 | - |
assay at, natural substrates | Bitis gabonica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
Tosyl-Gly-Pro-Arg 4-nitroanilide | - |
Bitis gabonica | |
0.065 | - |
H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide | - |
Bitis gabonica | |
0.204 | - |
tosyl-Gly-Pro-Lys 4-nitroanilide | - |
Bitis gabonica | |
0.248 | - |
H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide | - |
Bitis gabonica | |
0.952 | - |
H-D-Phenylglycine-Phe-Arg 4-nitroanilide | - |
Bitis gabonica | |
12.2 | - |
tosyl-L-Arg methyl ester | - |
Bitis gabonica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.3 | Bitis gabonica |