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Literature summary for 3.4.21.53 extracted from

  • Takeuchi, K.; Tsuchiya, W.; Noda, N.; Suzuki, R.; Yamazaki, T.; Haas, D.
    Lon protease negatively affects GacA protein stability and expression of the Gac/Rsm signal transduction pathway in Pseudomonas protegens (2014), Environ. Microbiol., 16, 2538-2549.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene PFL_3985 Pseudomonas protegens
gene PFL_3985, functional recombinant expression of His-tagged enzyme in Escherichia coli Pseudomonas protegens

Protein Variants

Protein Variants Comment Organism
additional information Pseudomonas protegens CHA1321(pycA-)/pME7402 is mutagenized by inserting transposon Tn5 in a mating with Escherichia coli W3110/pLG221. Complementation of the lon-negative mutant Pseudomonas protegens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas protegens

Organism

Organism UniProt Comment Textmining
Pseudomonas protegens A0A2C9ETQ7 formerly Pseudomonas fluorescens, gene PFL_3985
-
Pseudomonas protegens CHA0 A0A2C9ETQ7 formerly Pseudomonas fluorescens, gene PFL_3985
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration Pseudomonas protegens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-casein + H2O cleavage in an ATP-dependent manner Pseudomonas protegens ?
-
?
alpha-casein + H2O cleavage in an ATP-dependent manner Pseudomonas protegens CHA0 ?
-
?

Synonyms

Synonyms Comment Organism
lon protease
-
Pseudomonas protegens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas protegens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas protegens

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Pseudomonas protegens

General Information

General Information Comment Organism
evolution the enzyme is a member of the ATP-dependent protease family Pseudomonas protegens
malfunction in a lon mutant, the steady-state levels and the stability of the GacA protein are significantly elevated at the end of exponential growth, the expression of the sRNAs RsmY and RsmZ and that of dependent physiological functions such as antibiotic production are significantly enhanced. In starved cells, the loss of Lon function prolonged the half-life of the GacA protein. The lon mutant has a higher biocontrol activity per viable cell, but this positive effect appears to be compromised by a reduced fitness of the mutant in the rhizosphere on cucumber. Biocontrol of Pythium ultimum on cucumber roots requires fewer lon mutant cells than wild-type cells. In starved cells, the loss of Lon function prolonges the half-life of the GacA protein. The lon mutant exhibits increased aprA expression and antibiotic activity Pseudomonas protegens
physiological function ATP-dependent proteases, e.g. represented by Lon, are stress proteins that are induced in bacterial cells in response to unfavourable conditions. The enzyme negatively affects GacA protein stability and expression of the Gac/Rsm signal transduction pathway in Pseudomonas protegens, it is an important negative regulator of the Gac/Rsm signal transduction pathway in the organism. The Gac/Rsm signal transduction pathway controls secondary metabolism and suppression of fungal root pathogens via the expression of regulatory small RNAs, overview Pseudomonas protegens