Cloned (Comment) | Organism |
---|---|
gene PFL_3985 | Pseudomonas protegens |
gene PFL_3985, functional recombinant expression of His-tagged enzyme in Escherichia coli | Pseudomonas protegens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Pseudomonas protegens CHA1321(pycA-)/pME7402 is mutagenized by inserting transposon Tn5 in a mating with Escherichia coli W3110/pLG221. Complementation of the lon-negative mutant | Pseudomonas protegens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas protegens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas protegens | A0A2C9ETQ7 | formerly Pseudomonas fluorescens, gene PFL_3985 | - |
Pseudomonas protegens CHA0 | A0A2C9ETQ7 | formerly Pseudomonas fluorescens, gene PFL_3985 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration | Pseudomonas protegens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-casein + H2O | cleavage in an ATP-dependent manner | Pseudomonas protegens | ? | - |
? | |
alpha-casein + H2O | cleavage in an ATP-dependent manner | Pseudomonas protegens CHA0 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lon protease | - |
Pseudomonas protegens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas protegens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas protegens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Pseudomonas protegens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the ATP-dependent protease family | Pseudomonas protegens |
malfunction | in a lon mutant, the steady-state levels and the stability of the GacA protein are significantly elevated at the end of exponential growth, the expression of the sRNAs RsmY and RsmZ and that of dependent physiological functions such as antibiotic production are significantly enhanced. In starved cells, the loss of Lon function prolonged the half-life of the GacA protein. The lon mutant has a higher biocontrol activity per viable cell, but this positive effect appears to be compromised by a reduced fitness of the mutant in the rhizosphere on cucumber. Biocontrol of Pythium ultimum on cucumber roots requires fewer lon mutant cells than wild-type cells. In starved cells, the loss of Lon function prolonges the half-life of the GacA protein. The lon mutant exhibits increased aprA expression and antibiotic activity | Pseudomonas protegens |
physiological function | ATP-dependent proteases, e.g. represented by Lon, are stress proteins that are induced in bacterial cells in response to unfavourable conditions. The enzyme negatively affects GacA protein stability and expression of the Gac/Rsm signal transduction pathway in Pseudomonas protegens, it is an important negative regulator of the Gac/Rsm signal transduction pathway in the organism. The Gac/Rsm signal transduction pathway controls secondary metabolism and suppression of fungal root pathogens via the expression of regulatory small RNAs, overview | Pseudomonas protegens |