Application | Comment | Organism |
---|---|---|
additional information | lon protease functions in trans translation | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
C-terminally His6-tagged lon expressed in Escherichia coli strain CH1019 carrying lon expression plasmid pET21b-LonH6 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | in an endogenous protein tagging assay, lon mutants accumulate excessive levels of tmRNA-tagged proteins. In a reporter protein tagging assay with lambda-CI-N, lon mutants efficiently tag the reporter protein, but the tagged protein exhibits increased stability. GFP construct containing a hard-coded C-terminal tmRNA tag exhibits increased stability in lon mutant cells | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tmRNA-tagged protein + H2O | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 derivative W3110 [F- lambda- IN(rrnD-rrnE)1 rph-1] | - |
Purification (Comment) | Organism |
---|---|
by successive Ni2+-NTA affinity chromatography and anion-exchange chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | lon contains three distinct domains, an amino-terminal domain having an undefined function, a central ATPase domain crucial for substrate binding and unfolding, and a C-terminal peptidase domain | Escherichia coli | ? | - |
? | |
tmRNA-tagged protein + H2O | - |
Escherichia coli | ? | - |
? | |
tmRNA-tagged protein + H2O | highly purified lon preferentially degrades tmRNA-tagged forms of proteins compared to untagged forms | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lon | - |
Escherichia coli |
lon ATP-dependent protease | - |
Escherichia coli |
lon protease | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |