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Literature summary for 3.4.21.53 extracted from

  • Lee, A.Y.; Tsay, S.S.; Chen, M.Y.; Wu, S.H.
    Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme (2004), Eur. J. Biochem., 271, 834-844.
    View publication on PubMed
Search for more literature for 3.4.21.53

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
 Brevibacillus thermoruber

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 6 * 90000, SDS-PAGE, 6 * 88000, calculated Brevibacillus thermoruber
90000
-
 6 * 90000, SDS-PAGE, 6 * 88000, calculated Brevibacillus thermoruber
549000
-
 gel filtration Brevibacillus thermoruber

Organism

Organism UniProt Comment Textmining
Brevibacillus thermoruber Q84FG5 expression is heat-inducible. In addition to ATPase activity and peptidase activity, enzyme has an ATP-independent chaperone-like activity
-
Brevibacillus thermoruber WR-249 Q84FG5 expression is heat-inducible. In addition to ATPase activity and peptidase activity, enzyme has an ATP-independent chaperone-like activity
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
267.7
-
 substrate succinyl-FLF-4-methoxy-beta-naphthylamide, pH 8,0, 50°C Brevibacillus thermoruber
697.6
-
 substrate glutaryl-AAF-4-methoxy-beta-naphthylamide, pH 8,0, 50°C Brevibacillus thermoruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-AAF-4-methoxy-beta-naphthylamide + H2O preferred substrate Brevibacillus thermoruber glutaryl-L-Ala-L-Ala-L-Phe + 4-methoxy-2-naphthylamine
-
 ?
glutaryl-AAF-4-methoxy-beta-naphthylamide + H2O preferred substrate Brevibacillus thermoruber WR-249 glutaryl-L-Ala-L-Ala-L-Phe + 4-methoxy-2-naphthylamine
-
 ?
succinyl-FLF-4-methoxy-beta-naphthylamide + H2O
-
 Brevibacillus thermoruber succinyl-FLF + 4-methoxy-beta-naphthylamine
-
 ?
succinyl-FLF-4-methoxy-beta-naphthylamide + H2O
-
 Brevibacillus thermoruber WR-249 succinyl-FLF + 4-methoxy-beta-naphthylamine
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 90000, SDS-PAGE, 6 * 88000, calculated Brevibacillus thermoruber
More protein consists of an N-terminal domain, a central ATPase domain which includes a sensor- and substrate-discrimination domain, and a C-terminal protease domain Brevibacillus thermoruber

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 peptidase activity Brevibacillus thermoruber
70
-
 ATPase activity Brevibacillus thermoruber

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
71.5
-
 melting temperature Brevibacillus thermoruber