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Literature summary for 3.4.21.53 extracted from

  • Suzuki, C.K.; Kutejova, E.; Suda, K.
    Analysis and purification of ATP-dependent mitochondrial lon protease of Saccharomyces cerevisiae (1995), Methods Enzymol., 260, 486-494.
    View publication on PubMed
Search for more literature for 3.4.21.53

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate inhibition indicates that the enzyme is a serine protease Saccharomyces cerevisiae
N-ethylmaleimide
-
 Escherichia coli
N-ethylmaleimide enzyme particularly sensitive to, pointing to an essential role of sulfllydryl groups in enzymatic activity Saccharomyces cerevisiae
phenylmethylsulfonyl fluoride inhibition indicates that the enzyme is a serine protease Saccharomyces cerevisiae
PMSF
-
 Escherichia coli
PMSF
-
 Saccharomyces cerevisiae
vanadate
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence compared to other Lon-protein sequences Escherichia coli
additional information
-
 amino acid sequence compared to other Lon-protein sequences Saccharomyces cerevisiae
106000
-
 6 * 106000, human, calculated from amino acid sequence Escherichia coli
106000
-
 6 * 106000, human, calculated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli
-
 ?
-
 ?
additional information Saccharomyces cerevisiae involved in mitochondrial protein turnover ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae
-
 yeast, mutant strain pep949
-

Purification (Commentary)

Purification (Comment) Organism
protein purification procedure utilizes mitochondria from the mutant strain pep949 (MATa/alpha, prl 1-1122, prc 1-40Z, pep 4-3, leu2, trp 1, ura 3-52) Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O alpha-casein Escherichia coli hydrolyzed casein
-
 ?
casein + H2O alpha-casein Saccharomyces cerevisiae hydrolyzed casein
-
 ?
casein + H2O alpha-casein (alpha1-casein) Saccharomyces cerevisiae hydrolyzed casein
-
 ?
additional information
-
 Escherichia coli ?
-
 ?
additional information involved in mitochondrial protein turnover Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 106000, human, calculated from amino acid sequence Escherichia coli
hexamer 6 * 106000, human, calculated from amino acid sequence Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
lon protease
-
 Saccharomyces cerevisiae
protease
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP ATP-dependent protease Escherichia coli
ATP ATP-dependent protease Saccharomyces cerevisiae