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Literature summary for 3.4.21.53 extracted from

  • Waxman, L.; Goldberg, A.L.
    Selectivity of intracellular proteolysis: protein substrates activate the ATP-dependent protease (La) (1986), Science, 232, 500-503.
    View publication on PubMed
Search for more literature for 3.4.21.53

Activating Compound

Activating Compound Comment Organism Structure
Bovine glucagon activation, glutaryl-Ala-Ala-Phe methoxynaphthylamide hydrolysis, with or without ATP, not casein hydrolysis Escherichia coli
casein activation, glutaryl-Ala-Ala-Phe-methoxynaphthylamide or insulin B-chain hydrolysis, in the absence of ATP and synergistic with ATP Escherichia coli
Denatured bovine serum albumin activation Escherichia coli
Denatured bovine serum albumin glutaryl-Ala-Ala-Phe methoxynaphthylamide hydrolysis, with or without ATP Escherichia coli
Denatured immunoglobin G activation Escherichia coli
Denatured immunoglobin G peptide hydrolysis, with or without ATP Escherichia coli
Globin activation, peptide hydrolysis Escherichia coli
additional information no activation by nonhydrolyzable proteins, e.g. native or denatured ribonuclease or lysozyme Escherichia coli
Protein substrates activation Escherichia coli
Protein substrates rise in ATPase activity proportional to peptide bonds cleaved Escherichia coli
Protein substrates protein substrates enhance additively the stimulating effect of ATP on peptide hydrolysis and even in the absence of ATP they enhance the ability to degrade fluorogenic tripeptides Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information casein (with peptides as substrates) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 alpha-casein
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Proteins with highly abnormal conformation + H2O Escherichia coli rate-limiting step in breakdown of highly abnormal and some normal proteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
hydrolysis of proteins in presence of ATP hydrolysis of proteins in presence of ATP, mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-casein + H2O
-
 Escherichia coli ?
-
 ?
Denatured bovine serum albumin + H2O
-
 Escherichia coli ?
-
 ?
Denatured immunoglobulin G + H2O
-
 Escherichia coli ?
-
 ?
Globin + H2O
-
 Escherichia coli ?
-
 ?
additional information No substrates are native bovine serum albumin, hemoglobin Escherichia coli ?
-
 ?
additional information the active site prefers hydrophobic substrate sequences Escherichia coli ?
-
 ?
additional information No substrates are benzyloxycarbonyl-Ala-Arg-Arg-methoxynaphthylamide, native or denatured ribonuclease, native or denatured lysozyme, native immunoglobulin G Escherichia coli ?
-
 ?
Proteins with highly abnormal conformation + H2O rate-limiting step in breakdown of highly abnormal and some normal proteins Escherichia coli ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP ATP-dependent protease Escherichia coli