Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Porphyromonas gingivalis | |
pepstatin | slight activation | Porphyromonas gingivalis |
Cloned (Comment) | Organism |
---|---|
gene PGN_1416, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3) | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Porphyromonas gingivalis | |
chymostatin | - |
Porphyromonas gingivalis | |
leupeptin | - |
Porphyromonas gingivalis | |
additional information | no inhibition by pepstatin and EDTA | Porphyromonas gingivalis | |
N-tosyl-L-Lys chloromethyl ketone | TLCK | Porphyromonas gingivalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | the enzyme is secreted by the type IX secretion system, T9SS, and anchored onto the cell surface by binding to A-LPS | Porphyromonas gingivalis | 9986 | - |
extracellular | the enzyme is secreted by the type IX secretion system, T9SS, and anchored onto the cell surface by binding to A-LPS | Porphyromonas gingivalis | - |
- |
additional information | blockage of the type IX secretion system, T9SS, leads to localization of the enzyme in the outer membrane, subcellular localization study overview | Porphyromonas gingivalis | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | B2RKP0 | strain ATCC 33277; diverse strains, i.e. ATCC 33277, W83, TDC60, TDC117, TDC275, GAI7802, SU63 and HG66, gene PGN_1416 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the PGN_1416 protein activation requires processing by cysteine protease Rgp | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Boc-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O | - |
Porphyromonas gingivalis | Boc-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? | |
additional information | Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin is a poor substrate | Porphyromonas gingivalis | ? | - |
? | |
additional information | the enzyme has a Lys-specific serine endopeptidase activity and requires processing by cysteine protease Rgp for its activation | Porphyromonas gingivalis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lys-specific serine endopeptidase | - |
Porphyromonas gingivalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
assay at | Porphyromonas gingivalis |