Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.50 extracted from

  • Nonaka, M.; Shoji, M.; Kadowaki, T.; Sato, K.; Yukitake, H.; Naito, M.; Nakayama, K.
    Analysis of a Lys-specific serine endopeptidase secreted via the type IX secretion system in Porphyromonas gingivalis (2014), FEMS Microbiol. Lett., 354, 60-68.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
Porphyromonas gingivalis
pepstatin slight activation Porphyromonas gingivalis

Cloned(Commentary)

Cloned (Comment) Organism
gene PGN_1416, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3) Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
Porphyromonas gingivalis
chymostatin
-
Porphyromonas gingivalis
leupeptin
-
Porphyromonas gingivalis
additional information no inhibition by pepstatin and EDTA Porphyromonas gingivalis
N-tosyl-L-Lys chloromethyl ketone TLCK Porphyromonas gingivalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface the enzyme is secreted by the type IX secretion system, T9SS, and anchored onto the cell surface by binding to A-LPS Porphyromonas gingivalis 9986
-
extracellular the enzyme is secreted by the type IX secretion system, T9SS, and anchored onto the cell surface by binding to A-LPS Porphyromonas gingivalis
-
-
additional information blockage of the type IX secretion system, T9SS, leads to localization of the enzyme in the outer membrane, subcellular localization study overview Porphyromonas gingivalis
-
-

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis B2RKP0 strain ATCC 33277; diverse strains, i.e. ATCC 33277, W83, TDC60, TDC117, TDC275, GAI7802, SU63 and HG66, gene PGN_1416
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the PGN_1416 protein activation requires processing by cysteine protease Rgp Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
Porphyromonas gingivalis Boc-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
?
additional information Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin is a poor substrate Porphyromonas gingivalis ?
-
?
additional information the enzyme has a Lys-specific serine endopeptidase activity and requires processing by cysteine protease Rgp for its activation Porphyromonas gingivalis ?
-
?

Synonyms

Synonyms Comment Organism
Lys-specific serine endopeptidase
-
Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
assay at Porphyromonas gingivalis