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Literature summary for 3.4.21.50 extracted from

  • Malloy, J.L.; Veldhuizen, R.A.; Thibodeaux, B.A.; O'Callaghan, R.J.; Wright, J.R.
    Pseudomonas aeruginosa protease IV degrades surfactant proteins and inhibits surfactant host defense and biophysical functions (2005), Am. J. Physiol. Lung Cell Mol. Physiol., 288, L409-L418.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, about, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview ?
-
?
additional information Pseudomonas aeruginosa PA103-29 the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa PA103-29
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain PA103-29 by ion exchange chromatography and gel filtration, or from strain PAO1-B1 supernatants by ultrafiltration, anion exchange and hydrophobic interaction chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
pathogen-host surface activity analysis, overview Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview Pseudomonas aeruginosa ?
-
?
additional information the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview Pseudomonas aeruginosa PA103-29 ?
-
?
tosyl-Gly-Pro-Lys-4-nitroanilide + H2O a chromogenic substrate Pseudomonas aeruginosa tosyl-Gly-Pro-Lys + 4-nitroaniline
-
?
tosyl-Gly-Pro-Lys-4-nitroanilide + H2O a chromogenic substrate Pseudomonas aeruginosa PA103-29 tosyl-Gly-Pro-Lys + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
? x * 30000, about, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
protease IV
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 37 assay at Pseudomonas aeruginosa