Cloned (Comment) | Organism |
---|---|
- |
Lysobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | - |
Lysobacter sp. | |
Guanidine-HCl | 0.1 M, 20 min, 72% loss of activity | Lysobacter sp. | |
N-tosyl-L-Lys chloromethylketone | - |
Lysobacter sp. | |
sodium dodecylsulfate | 0.1%, 20 min, 70% loss of activity | Lysobacter sp. | |
Urea | 4 M, 20 min, 80% loss of activity | Lysobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Lysobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysobacter sp. | Q8GQZ0 | isoform LepB | - |
Lysobacter sp. IB-9374 | Q8GQZ0 | isoform LepB | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a prepetide of 20 amino acids, a propeptide of 184 amino acids and a mature enzyme of 274 amino acids with a C-terminal extension peptide of 200 amino acids | Lysobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.3 | - |
pH 9.2, 30°C | Lysobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-L-Lys-4-nitroanilide + H2O | - |
Lysobacter sp. | benzoyl-L-Lys + 4-nitroaniline | - |
? | |
benzoyl-L-Lys-4-nitroanilide + H2O | - |
Lysobacter sp. IB-9374 | benzoyl-L-Lys + 4-nitroaniline | - |
? | |
Glucagon + H2O | specific hydrolysis of lysyl peptide bond | Lysobacter sp. | ? | - |
? | |
Glucagon + H2O | specific hydrolysis of lysyl peptide bond | Lysobacter sp. IB-9374 | ? | - |
? | |
L-Lys-4-nitroanilide + H2O | slow reaction | Lysobacter sp. | L-Lys + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | slow reaction | Lysobacter sp. IB-9374 | L-Lys + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Lysobacter sp. |
More | isozyme of LepA, can fold into a three-dimensional structure quite similar to LepA | Lysobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lysobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
moderately stable up to | Lysobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | more than 50% of maximum activity in this range | Lysobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | 4°C, stable for at least 24 h | Lysobacter sp. |