Protein Variants | Comment | Organism |
---|---|---|
H210A | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 7.1% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is identical to the wild-type value | Achromobacter lyticus |
H210F | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 14% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 29.5% of the wild-type value | Achromobacter lyticus |
H210S | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 8.6% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 1.7fold higher than the wild-type value | Achromobacter lyticus |
W169A | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 0.5% of the wild-type value | Achromobacter lyticus |
W169F | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 45% of the wild-type value | Achromobacter lyticus |
W169G | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 0.06% of the wild-type value | Achromobacter lyticus |
W169H | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 9% of the wild-type value | Achromobacter lyticus |
W169L | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 25% of the wild-type value | Achromobacter lyticus |
W169V | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 6% of the wild-type value | Achromobacter lyticus |
W169Y | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 43% of the wild-type value | Achromobacter lyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210S | Achromobacter lyticus | |
0.0013 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210A | Achromobacter lyticus | |
0.0021 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, wild-type enzyme | Achromobacter lyticus | |
0.0028 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, wild-type enzyme | Achromobacter lyticus | |
0.0034 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169L | Achromobacter lyticus | |
0.0036 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169F | Achromobacter lyticus | |
0.0037 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, wild-type enzyme | Achromobacter lyticus | |
0.004 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169F | Achromobacter lyticus | |
0.0043 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169Y | Achromobacter lyticus | |
0.0046 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169L | Achromobacter lyticus | |
0.0058 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169F | Achromobacter lyticus | |
0.006 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210S | Achromobacter lyticus | |
0.0061 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210A | Achromobacter lyticus | |
0.0063 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169Y | Achromobacter lyticus | |
0.0072 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169Y | Achromobacter lyticus | |
0.0073 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210A | Achromobacter lyticus | |
0.0099 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169L | Achromobacter lyticus | |
0.01 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210A | Achromobacter lyticus | |
0.01 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169V | Achromobacter lyticus | |
0.012 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169V | Achromobacter lyticus | |
0.017 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169H | Achromobacter lyticus | |
0.02 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169A | Achromobacter lyticus | |
0.023 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169A | Achromobacter lyticus | |
0.024 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169H | Achromobacter lyticus | |
0.029 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169F | Achromobacter lyticus | |
0.032 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169G | Achromobacter lyticus | |
0.034 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169A | Achromobacter lyticus | |
0.034 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169H | Achromobacter lyticus | |
0.037 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169Y | Achromobacter lyticus | |
0.038 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, wild-type enzyme | Achromobacter lyticus | |
0.042 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169V | Achromobacter lyticus | |
0.046 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169L | Achromobacter lyticus | |
0.066 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169A | Achromobacter lyticus | |
0.074 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169H | Achromobacter lyticus | |
0.081 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169V | Achromobacter lyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter lyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin + H2O | - |
Achromobacter lyticus | tert-butyloxycarbonyl-Ala-Ala-Lys + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin + H2O | - |
Achromobacter lyticus | tert-butyloxycarbonyl-Ala-Lys + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin + H2O | - |
Achromobacter lyticus | tert-butyloxycarbonyl-Lys + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O | - |
Achromobacter lyticus | tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169A | Achromobacter lyticus | |
0.8 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169G | Achromobacter lyticus | |
1.1 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169A | Achromobacter lyticus | |
2 | 8 | tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169V | Achromobacter lyticus | |
2.3 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169V | Achromobacter lyticus | |
2.6 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169A | Achromobacter lyticus | |
2.7 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169H | Achromobacter lyticus | |
3 | 6 | tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169L | Achromobacter lyticus | |
4.5 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169L | Achromobacter lyticus | |
4.6 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169A | Achromobacter lyticus | |
5.2 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169Y | Achromobacter lyticus | |
6 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169F | Achromobacter lyticus | |
7 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169V | Achromobacter lyticus | |
8.4 | - |
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, wild-type enzyme | Achromobacter lyticus | |
11 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169H | Achromobacter lyticus | |
15 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210A | Achromobacter lyticus | |
18 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210S | Achromobacter lyticus | |
20 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169V | Achromobacter lyticus | |
22 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169Y | Achromobacter lyticus | |
23 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169H | Achromobacter lyticus | |
24 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169F | Achromobacter lyticus | |
31 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169L | Achromobacter lyticus | |
37 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169L | Achromobacter lyticus | |
40 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169Y | Achromobacter lyticus | |
45 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210A | Achromobacter lyticus | |
46 | - |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, wild-type enzyme | Achromobacter lyticus | |
48 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169F | Achromobacter lyticus | |
51 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210A | Achromobacter lyticus | |
52 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210S | Achromobacter lyticus | |
67 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169H | Achromobacter lyticus | |
71 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169F | Achromobacter lyticus | |
83 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme W169Y | Achromobacter lyticus | |
93 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, wild-type enzyme | Achromobacter lyticus | |
96 | - |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, mutant enzyme H210A | Achromobacter lyticus | |
98 | - |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin | pH 9.0, 37°C, wild-type enzyme | Achromobacter lyticus |