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Literature summary for 3.4.21.50 extracted from

  • Chohnan, S.; Nonaka, J.; Teramoto, K.; Taniguchi, K.; Kameda, Y.; Tamura, H.; Kurusu, Y.; Norioka, S.; Masaki, T.; Sakiyama, F.
    Lysobacter strain with high lysyl endopeptidase production (2002), FEMS Microbiol. Lett., 213, 13-20.
    View publication on PubMed
Search for more literature for 3.4.21.50

Cloned(Commentary)

Cloned (Comment) Organism
-
 Lysobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM, 24% inhibition of amidolytic activity Lysobacter sp.
diisopropyl fluorophosphate 10 mM, almost complete inhibition of amidolytic activity Lysobacter sp.
Nalpha-p-tosyl-L-Lys-chloromethylketone 0.1 mM, almost complete inhibition of amidolytic activity Lysobacter sp.
phenylmethanesulfonyl fluoride 2 mM, almost complete inhibition of amidolytic activity Lysobacter sp.
Zn2+ 1 mM, 34% inhibition of amidolytic activity Lysobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Lysobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 gel filtration Lysobacter sp.
27850
-
 ion-spray mass spectrometry Lysobacter sp.
31000
-
 1 * 31000, SDS-PAGE Lysobacter sp.

Organism

Organism UniProt Comment Textmining
Lysobacter sp. Q9AQR5
-
-
Lysobacter sp. IB-9374 Q9AQR5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the total precursor protein is 671 amino acids of which only 268 amino acids are in the finally processed exoenzyme Lysobacter sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Lysobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Lysobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6
-
-
 Lysobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Lys-p-nitroaniline + H2O
-
 Lysobacter sp. acetyl-Lys + p-nitroaniline
-
 ?
amyloid beta-protein + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. ?
-
 ?
amyloid beta-protein + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. IB-9374 ?
-
 ?
benzoyl-Lys methyl ester + H2O
-
 Lysobacter sp. benzoyl-Lys + methanol
-
 ?
benzoyl-Lys methyl ester + H2O
-
 Lysobacter sp. IB-9374 benzoyl-Lys + methanol
-
 ?
benzoyl-Lys-p-nitroanilide + H2O
-
 Lysobacter sp. benzoyl-Lys + p-nitroaniline
-
 ?
benzoyl-Lys-p-nitroaniline + H2O
-
 Lysobacter sp. benzoyl-Lys + p-nitroaniline
-
 ?
benzoyl-Orn methyl ester + H2O
-
 Lysobacter sp. benzoyl-Orn + methanol
-
 ?
Glucagon + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. ?
-
 ?
Glucagon + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. IB-9374 ?
-
 ?
additional information no activity with benzoyl-Arg-p-nitroanilide, Arg-p-nitroanilide, succinyl-Ala-p-nitroanilide, tert-butyloxycarbonyl-His-p-nitroanilide, acetyl-Phe-p-nitroanilide, benzoyl-Tyr-p-nitroanilide and acetyl-Leu-p-nitroanilide Lysobacter sp. ?
-
 ?
additional information no activity with benzoyl-Arg-p-nitroanilide, Arg-p-nitroanilide, succinyl-Ala-p-nitroanilide, tert-butyloxycarbonyl-His-p-nitroanilide, acetyl-Phe-p-nitroanilide, benzoyl-Tyr-p-nitroanilide and acetyl-Leu-p-nitroanilide Lysobacter sp. IB-9374 ?
-
 ?
oxidized insulin B chain + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. ?
-
 ?
oxidized insulin B chain + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. IB-9374 ?
-
 ?
tosyl-Lys methyl ester + H2O
-
 Lysobacter sp. tosyl-Lys + methanol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Lysobacter sp.

Synonyms

Synonyms Comment Organism
LEP
-
 Lysobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 hydrolysis of benzoyl-Lys-p-nitroanilide Lysobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 8.0, 30°C, stable Lysobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 esterolytic activity Lysobacter sp.
8.5 10.5 caseinolytic activity Lysobacter sp.
9 9.6 amidolytic activity Lysobacter sp.

pI Value

Organism Comment pI Value Maximum pI Value
Lysobacter sp. isoelectric focusing
-
 6.8