Cloned (Comment) | Organism |
---|---|
the cloned gene of prepro-API is expressed in Escherichia coli, pro-API is secreted into the periplasm and activated autocatalytically | Achromobacter lyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-amino-1-hexanoic acid | - |
Achromobacter lyticus | |
acetonitrile | - |
Achromobacter lyticus | |
Ba2+ | - |
Achromobacter lyticus | |
benzoyl-DL-lysinal | - |
Achromobacter lyticus | |
Benzoyl-Lys | - |
Achromobacter lyticus | |
benzyloxycarbonyl-Leu-Leu-lysinal | - |
Achromobacter lyticus | |
benzyloxycarbonyl-Pro-lysinal | - |
Achromobacter lyticus | |
benzyloxycarbonyl-Val-lysinal | - |
Achromobacter lyticus | |
Cs+ | - |
Achromobacter lyticus | |
Cyclohexylamine | - |
Achromobacter lyticus | |
diisopropylfluorophosphate | - |
Achromobacter lyticus | |
ethylamine | - |
Achromobacter lyticus | |
hydrazine | - |
Achromobacter lyticus | |
Isobutylamine | - |
Achromobacter lyticus | |
K+ | - |
Achromobacter lyticus | |
Li+ | - |
Achromobacter lyticus | |
Lys | - |
Achromobacter lyticus | |
methylamine | - |
Achromobacter lyticus | |
n-alkylamine | - |
Achromobacter lyticus | |
n-amylamine | - |
Achromobacter lyticus | |
n-butylamine | - |
Achromobacter lyticus | |
n-Hexylamine | - |
Achromobacter lyticus | |
n-Propylamine | - |
Achromobacter lyticus | |
Na+ | - |
Achromobacter lyticus | |
Nalpha-p-tosyl-L-Lys-chloromethylketone | - |
Achromobacter lyticus | |
NH4+ | - |
Achromobacter lyticus | |
phenylmethanesulfonyl fluoride | - |
Achromobacter lyticus | |
Rb+ | - |
Achromobacter lyticus | |
Zn2+ | - |
Achromobacter lyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
Trypsinogen | - |
Achromobacter lyticus | |
0.07 | - |
N-benzoyl-L-Lys p-nitroanilide | - |
Achromobacter lyticus | |
0.08 | - |
Lys-p-nitroanilide | - |
Achromobacter lyticus | |
0.091 | - |
benzoyl-Lys methyl ester | - |
Achromobacter lyticus | |
0.1 | - |
N-tosyl-L-Lys methyl ester | - |
Achromobacter lyticus | |
0.32 | - |
benzoyl-Lys-NH2 | - |
Achromobacter lyticus | |
1 | - |
N-benzoyl-L-Orn methyl ester | - |
Achromobacter lyticus | |
3.09 | - |
N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
3.11 | - |
N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
35.7 | - |
N-tosyl-L-Arg methyl ester | - |
Achromobacter lyticus | |
41 | - |
N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 20%, 37°C, enzyme retains most of its activity | Achromobacter lyticus |
Ethanol | 20%, 37°C, enzyme retains most of its activity | Achromobacter lyticus |
Methanol | 20%, 37°C, enzyme retains most of its activity | Achromobacter lyticus |
SDS | 0.1% w/v, in 40 mM Tris-HCl, pH 8, 20 min, 30°C, stable | Achromobacter lyticus |
urea | 4 M, stable | Achromobacter lyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter lyticus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the cloned gene of prepro-API is expressed in E. coli, pro-API is secreted into the periplasm and activated autocatalytically | Achromobacter lyticus |
Purification (Comment) | Organism |
---|---|
- |
Achromobacter lyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Achromobacter lyticus |
Storage Stability | Organism |
---|---|
-20°C, 0.001-0.1 M Tris-HCl buffer, pH 6.0-10.9, at enzyme concentration higher than 0.1 mg/ml | Achromobacter lyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Lys methyl ester + H2O | - |
Achromobacter lyticus | benzoyl-Lys + methanol | - |
? | |
benzoyl-Lys-NH2 + H2O | - |
Achromobacter lyticus | benzoyl-Lys + NH3 | - |
? | |
benzoyl-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | benzoyl-Lys + p-nitroaniline | - |
? | |
Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | Lys + p-nitroaniline | - |
? | |
N-benzoyl-L-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Lys + p-nitroaniline | - |
? | |
N-benzoyl-L-Orn methyl ester + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Orn + methanol | - |
? | |
N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide + H2O | - |
Achromobacter lyticus | N-tert-butyloxycarbonyl-Ala-Ala-Lys + 7-amino-4-methylcoumarin | - |
? | |
N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide + H2O | - |
Achromobacter lyticus | N-tert-butyloxycarbonyl-Ala-Lys + 7-amino-4-methylcoumarin | - |
? | |
N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O | - |
Achromobacter lyticus | N-tert-butyloxycarbonyl-Lys + 7-amino-4-methylcoumarin | - |
? | |
N-tosyl-L-Arg methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Arg + methanol | - |
? | |
N-tosyl-L-Lys methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Lys + methanol | - |
? | |
Trypsinogen + H2O | - |
Achromobacter lyticus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable | Achromobacter lyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.103 | - |
Trypsinogen | - |
Achromobacter lyticus | |
0.14 | - |
Lys-p-nitroanilide | - |
Achromobacter lyticus | |
0.86 | - |
benzoyl-Lys-p-nitroanilide | - |
Achromobacter lyticus | |
1.54 | - |
benzoyl-Lys-NH2 | - |
Achromobacter lyticus | |
3.02 | - |
N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
5.04 | - |
N-tosyl-L-Arg methyl ester | - |
Achromobacter lyticus | |
10.4 | - |
N-benzoyl-L-Orn methyl ester | - |
Achromobacter lyticus | |
32.9 | - |
N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
49 | - |
N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
225 | - |
benzoyl-Lys methyl ester | - |
Achromobacter lyticus | |
570 | - |
N-tosyl-L-Lys methyl ester | - |
Achromobacter lyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
Achromobacter lyticus |
8.5 | 10.7 | caseinolytic activity | Achromobacter lyticus |
9 | 9.5 | - |
Achromobacter lyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | stable | Achromobacter lyticus |