Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.50 extracted from

  • Fujimura, S.; Shibata, Y.; Nakamura, T.
    Purification and partial characterization of a lysine-specific protease of Porphyromonas gingivalis (1993), FEMS Microbiol. Lett., 113, 133-138.
    View publication on PubMed
Search for more literature for 3.4.21.50

Activating Compound

Activating Compound Comment Organism Structure
EDTA activates Porphyromonas gingivalis
EGTA activates Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
antipain slight Porphyromonas gingivalis
Nalpha-p-tosyl-L-Lys-chloromethylketone
-
 Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 tosyl-Gly-Pro-Lys-p-nitroanilide
-
 Porphyromonas gingivalis
0.33
-
 Val-Leu-Lys-p-nitroanilide
-
 Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, SDS-PAGE Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Porphyromonas gingivalis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis N-benzoyl-L-Lys + p-nitroaniline
-
 ?
N-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis N-tosyl-Gly-Pro-Lys + p-nitroaniline
-
 ?
tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis tosyl-Gly-Pro-Lys + p-nitroaniline
-
 ?
Val-Leu-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis Val-Leu-Lys + p-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Porphyromonas gingivalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6 10 pH 6: about 50% of maximal activity, pH 10: about 55% of maximal activity Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Porphyromonas gingivalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 pH 7.5, 20 min, about 60% loss of activity Porphyromonas gingivalis
6.5 8.5 pH 7.5, 20 min, stable Porphyromonas gingivalis
10
-
 40% loss of activity Porphyromonas gingivalis