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Literature summary for 3.4.21.5 extracted from

  • Gallwitz, M.; Enoksson, M.; Thorpe, M.; Hellman, L.
    The extended cleavage specificity of human thrombin (2012), PLoS ONE, 7, e31756.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information determination of the extended substrate recognition profile. The consensus recognition sequence is, P2-Pro, P1-Arg, P19-Ser/Ala/Gly/Thr, P29-not acidic and P39-Arg. Residue P39-arginine in thrombin substrates lacking a P2-proline plays an important role. Upon insertion of the consensus sequence obtained in a linker region between two Escherichia coli thioredoxin molecules, mutations of P2-Pro and P39-Arg lead to an approximate 20fold and 14fold reduction, respectively in the rate of cleavage. Mutating both Pro and Arg results in a drop in cleavage of 200-400 times. No natural substrates display the obtained consensus sequence but represent sequences that show only 1-30% of the optimal cleavage rate for thrombin. Major effects on cleavage efficiency are also observed for residues as far away as 4 amino acids from the cleavage site. Insertion of an aspartic acid in position P4 results in a drop in cleavage by a factor of almost 20 times Homo sapiens ?
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