Literature summary for 3.4.21.5 extracted from

Russo Krauss, I.; Merlino, A.; Randazzo, A.; Mazzarella, L.; Sica, F.
Crystallization and preliminary X-ray analysis of the complex of human alpha-thrombin with a modified thrombin-binding aptamer (2010), Acta Crystallogr. Sect. F, 66, 961-963.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified alpha-thrombin complexed with a modified 15-mer thrombin-binding aptamer, sitting drop vapour diffusion method, 8 mg/ml protein in 50 mM potassium phosphate, pH 7.1, and 0.1 M KCl, is mixed in a 1:1 ratio of 0.001 ml each with crystallization solution, containing 20% v/v PEG 20 000, 0.2 M ammonium sulfate, 3% v/v n-propanol, 100 mM sodium acetate, pH 5.8, 20°C, a few days, X-ray diffraction structure determination and analysis at 2.15 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified alpha-thrombin complexed with a modified 15-mer thrombin-binding aptamer, sitting drop vapour diffusion method, 8 mg/ml protein in 50 mM potassium phosphate, pH 7.1, and 0.1 M KCl, is mixed in a 1:1 ratio of 0.001 ml each with crystallization solution, containing 20% v/v PEG 20 000, 0.2 M ammonium sulfate, 3% v/v n-propanol, 100 mM sodium acetate, pH 5.8, 20°C, a few days, X-ray diffraction structure determination and analysis at 2.15 A resolution

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
thrombin-binding aptamer	a consensus DNA 15-mer that binds specifically to human alpha-thrombin at nanomolar concentrations and inhibits its procoagulant functions. A a modified thrombin-binding aptamer, containing a 5'-5' inversion-of-polarity site, is more stable and to possesses a higher thrombin affinity than its unmodified counterpart	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Synonyms

Synonyms	Comment	Organism
alpha-thrombin	-	Homo sapiens

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Release 2023.1 (January 2023)