Literature summary for 3.4.21.5 extracted from

Iyaguchi, D.; Yao, M.; Watanabe, N.; Tanaka, I.; Toyota, E.
Crystallization and preliminary x-ray studies of the unliganded wild-type bovine thrombin (2007), Protein Pept. Lett., 14, 923-924.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, hanging drop vapour diffusion metod, 20°C, 0.002 ml of protein in 10 mM Tris-HCl, pH 8.0, and 50 mM NaCl, is mixed with 0.002 ml of reservoir solution containing 0.1 M sodium citrate, pH 5.6, 14-16% w/v PEG 4000, and 20% w/v 2-propanol, and equilibration against 0.1 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement method	Bos taurus

Crystallization (Comment)

Organism

purified enzyme, hanging drop vapour diffusion metod, 20°C, 0.002 ml of protein in 10 mM Tris-HCl, pH 8.0, and 50 mM NaCl, is mixed with 0.002 ml of reservoir solution containing 0.1 M sodium citrate, pH 5.6, 14-16% w/v PEG 4000, and 20% w/v 2-propanol, and equilibration against 0.1 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement method

Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
activated protein C	activated protein C has a regulatory function in inhibiting thrombin activation, overview	Bos taurus
Thrombomodulin	complex formation on endothelial cell surfaces blocks thrombin activity	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
factor V + H2O	Bos taurus	activation	activated factor V + ?	-	?
factor VIII + H2O	Bos taurus	activation	activated factor VIII + ?	-	?
factor XI + H2O	Bos taurus	activation	activated factor XI + ?	-	?
factor XII + H2O	Bos taurus	activated factor XII cross-links fibrin molecules and stabilizes the fibrin clot	activated factor XII + ?	-	?
fibrinogen + H2O	Bos taurus	the enzyme mediates the conversion of fibrinogen to fibrin	fibrin + ?	-	?
additional information	Bos taurus	thrombin is a multifunctional trypsin-like protease that plays a role in the blood coagulation system, stimulates platelet aggregation, and promotes its own generation through the activation of factor XI and cofactors V and VII	?	-	?
protease-activated receptor + H2O	Bos taurus	activation	?	-	?
protein C zymogen + H2O	Bos taurus	activated protein C has a regulatory function in inhibiting thrombin activation, overview	activated protein C + propeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
factor V + H2O	activation	Bos taurus	activated factor V + ?	-	?
factor VIII + H2O	activation	Bos taurus	activated factor VIII + ?	-	?
factor XI + H2O	activation	Bos taurus	activated factor XI + ?	-	?
factor XII + H2O	-	Bos taurus	activated factor XII + ?	-	?
factor XII + H2O	activated factor XII cross-links fibrin molecules and stabilizes the fibrin clot	Bos taurus	activated factor XII + ?	-	?
fibrinogen + H2O	-	Bos taurus	fibrin + ?	-	?
fibrinogen + H2O	the enzyme mediates the conversion of fibrinogen to fibrin	Bos taurus	fibrin + ?	-	?
additional information	thrombin is a multifunctional trypsin-like protease that plays a role in the blood coagulation system, stimulates platelet aggregation, and promotes its own generation through the activation of factor XI and cofactors V and VII	Bos taurus	?	-	?
protease-activated receptor + H2O	activation	Bos taurus	?	-	?
protease-activated receptor + H2O	activation	Bos taurus	activated protease-activated receptor + ?	-	?
protein C zymogen + H2O	activation	Bos taurus	activated protein C + propeptide	-	?
protein C zymogen + H2O	activated protein C has a regulatory function in inhibiting thrombin activation, overview	Bos taurus	activated protein C + propeptide	-	?